| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,734,269 | 0 | T | G | 51.5% | 22.8 / 44.3 | 33 | V402G (GTG→GGG) | avtA | valine‑‑pyruvate transaminase |
| Reads supporting (aligned to +/- strand): ref base T (3/13); new base G (17/0); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.77e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734185‑3734358 | cAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGaaaa > 1:414392/1‑88 (MQ=255) aGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGAt > 1:96714/1‑90 (MQ=255) ggATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGc > 2:312572/1‑90 (MQ=255) aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag < 1:50232/90‑1 (MQ=255) aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag < 2:438996/90‑1 (MQ=255) aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag < 1:312572/90‑1 (MQ=255) aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag < 1:408361/90‑1 (MQ=255) gTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGAt < 1:104932/90‑1 (MQ=255) tGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGGAGATTCTGGGGGGAGAGAtt > 2:345590/1‑89 (MQ=255) tGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTTGCGGGAGAGATa > 2:292581/1‑90 (MQ=255) tGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATa > 2:252033/1‑90 (MQ=255) cGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCt < 1:252033/90‑1 (MQ=255) aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTg > 2:84125/1‑90 (MQ=255) aTGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGt < 2:362446/90‑1 (MQ=255) aTGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGt > 1:190326/1‑90 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTTTGGGGGGAGGGGTAGAAAAAGCCCGGGCTGGAAGtt > 2:66615/1‑89 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTc > 1:49710/1‑90 (MQ=255) gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAAAGCCTGGGCTGAAAGTCa > 2:68969/1‑90 (MQ=255) aTGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGCAAAAATAGAAAAAGCCTGGGGCGAAAGTCACTaa > 2:118326/1‑90 (MQ=255) aTGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGGGATAGAAAGAGCCTGGGGTGAAAGTCACTaa > 1:28692/1‑90 (MQ=255) aCGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaaa < 1:410087/60‑1 (MQ=255) aCGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGAAAAAAAAAACCTGGGGTGGAAGTCAATAAAAAATGc > 2:397403/1‑90 (MQ=255) aCGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGaaa > 2:410087/1‑60 (MQ=255) aCCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGa < 1:292581/90‑1 (MQ=255) gagCCGGAGAAAATTGAGGCGGGGGGGAAGATTTTGGCGGGAGAGATAGAAAGAGGCTGGGGTGGAAGAGACTAAAAAATGCCTGATgcg > 2:389490/1‑90 (MQ=255) gagCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcg > 2:227425/1‑90 (MQ=255) agCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcgc < 2:336942/90‑1 (MQ=255) gCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCt > 1:8417/1‑90 (MQ=255) ggAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGGTGAAAGTCACTAAAAATTGCCTGATGCGCTACg > 2:141347/1‑90 (MQ=255) gagaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCTCTAAAAATTGCCTGATGCGCTACGc < 1:389490/90‑1 (MQ=255) aGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCt < 2:153300/42‑1 (MQ=255) aGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCt > 1:153300/1‑42 (MQ=255) gggggTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa < 1:84125/90‑1 (MQ=255) tGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAtt < 1:128258/90‑1 (MQ=255) | CAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734185‑3734358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |