| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,186,009 | 0 | T | G | 54.5% | 6.5 / 10.6 | 11 | T7P (ACC→CCC) | thiC | phosphomethylpyrimidine synthase ThiC |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATC > NZ_CP009273/4185923‑4186088 | gATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAg > 1:98237/1‑90 (MQ=255) tataAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAggt > 1:417476/1‑88 (MQ=255) gCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGc < 1:389042/90‑1 (MQ=255) gTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCCGTTTTTTTGGAg > 2:43921/1‑90 (MQ=255) gtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTc > 1:202347/1‑45 (MQ=255) gtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTc < 2:202347/45‑1 (MQ=255) gtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTTCAGACATAGCTCATTTCAAAAAAATAAAGACGTgggt > 1:421617/1‑88 (MQ=255) tCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTTCAGACATAGCTCATTCCCAAAAGGTAAGGACGGGGGtt > 1:411785/1‑90 (MQ=255) ggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGAt < 1:271805/90‑1 (MQ=255) gttCGCGGCGGGGCAGTTTTTTTTCAGACATAGCTCATTTCAAAAAAATAAGGGCGGGGGTTGTTTGACGACGGGTGGAGCAAAAGAGGa > 2:11572/1‑90 (MQ=255) tCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATc < 1:260136/90‑1 (MQ=255) | GATATAAATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATC > NZ_CP009273/4185923‑4186088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |