Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,884	C→A	P21Q (CCG→CAG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,884	0	C	A	100.0%	47.3 / NA	16	P21Q (CCG→CAG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/10);  total (6/10)

TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAC  >  NZ_CP009273/49801‑49937                                                                                    |                                                      ttttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGaa                                                 <  1:28942/90‑1 (MQ=255)   ttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAAcc                                               >  2:17722/1‑90 (MQ=255)      tttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAAcctgc                                            <  2:197468/90‑1 (MQ=255)                       aTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTgg                           >  2:119211/1‑90 (MQ=255)                            cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                      >  1:13317/1‑90 (MQ=255)                              gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGGGGAACCTGCCTGGCGATCTCGCCTGGTTTAAAc                    >  2:358409/1‑90 (MQ=255)                                  gATTGCGGCGTTGGCGGTAGACCGCGTTATCGGCATGAAAACCGCCAGCCAGTGGAACCTCCCTGCCGATCTCGCCTGGTTTAAACGCaa                <  2:279589/90‑1 (MQ=255)                                  gATTGCGGCGTTAGCGGTAGGTCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                <  1:179831/90‑1 (MQ=255)                                  gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                <  1:119211/90‑1 (MQ=255)                                  gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                <  1:167505/90‑1 (MQ=255)                                   aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac               <  1:269502/90‑1 (MQ=255)                                   aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac               <  2:13317/90‑1 (MQ=255)                                        ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                <  1:378826/84‑1 (MQ=255)                                        ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                >  2:378826/1‑84 (MQ=255)                                        ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGGTTAAACGCAACACCtt          >  2:285304/1‑90 (MQ=255)                                                cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc  <  2:124160/90‑1 (MQ=255)                                                                                    |                                                      TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAC  >  NZ_CP009273/49801‑49937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑