| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,171,725 | G→C | D185H (GAC→CAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,171,725 | 0 | G | C | 95.0% | 54.4 / ‑3.5 | 20 | D185H (GAC→CAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (10/9); minor base T (1/0); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
CCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCC > NZ_CP009273/4171648‑4171809 | ccGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCg > 2:115943/1‑90 (MQ=255) cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATcc < 1:64913/90‑1 (MQ=255) ggTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTCCCTGGCTGCACTTCGAATTCGATCCGAAg > 1:299509/1‑90 (MQ=255) aaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACaa < 1:205513/90‑1 (MQ=255) cccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc < 2:266492/90‑1 (MQ=255) ttCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTcgt < 2:78391/90‑1 (MQ=255) ttCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTcgt < 1:21151/90‑1 (MQ=255) ggTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTcgtacgt > 1:240816/1‑90 (MQ=255) aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTa > 1:176394/1‑90 (MQ=255) aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTa > 2:300871/1‑90 (MQ=255) aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTa > 2:202459/1‑90 (MQ=255) gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGTACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTaaa > 1:173048/1‑90 (MQ=255) gcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAAct < 1:418603/90‑1 (MQ=255) tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAAc > 1:398692/1‑76 (MQ=255) tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAAc < 2:398692/76‑1 (MQ=255) tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACcatc > 1:97200/1‑90 (MQ=255) tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACcatc > 2:342445/1‑90 (MQ=255) ggTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAAc < 1:318346/70‑1 (MQ=255) ggTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAAc > 2:318346/1‑70 (MQ=255) tCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc < 1:95664/90‑1 (MQ=255) tggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGcc > 1:216424/1‑90 (MQ=255) tggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGcc > 2:47428/1‑90 (MQ=255) ggctgtACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc < 2:173048/90‑1 (MQ=255) ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc < 1:202459/90‑1 (MQ=255) ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc < 2:362985/90‑1 (MQ=255) | CCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCC > NZ_CP009273/4171648‑4171809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |