| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 687,303 | 0 | A | G | 41.0% | 89.2 / 28.7 | 79 | intergenic (‑63/+27) | corC/ybeY | CNNM family magnesium/cobalt transport protein CorC/rRNA maturation RNase YbeY |
| Reads supporting (aligned to +/- strand): ref base A (9/37); new base G (32/0); total (42/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGT > NZ_CP009273/687219‑687386 | tgtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCt > 2:1426592/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:424145/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:2087942/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 1:2468950/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 1:1736486/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTg > 1:890671/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTg > 2:523658/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTg > 2:1522157/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGGCTg > 1:197907/1‑90 (MQ=255) gtgtgAATTGTCGTCGCGCATGGCGTCGTATGGGGTCTCATGTTAGGTAATCAAAACGCCGTCGGTAATCACCAACGGGGGTGTCTTCTg > 2:1700823/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGcc > 1:1178496/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGcc > 1:1617296/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGcc > 1:945941/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACC‑ACGGCGGGGGCGTCTGCCa > 1:2224609/1‑90 (MQ=255) tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCa > 1:1675558/1‑90 (MQ=255) cgtcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGCCAGGCaaaag > 2:240993/1‑88 (MQ=255) cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg < 1:721993/90‑1 (MQ=255) aTGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGTCTGCCCGTCAAAAGCCTGGcta > 1:2461553/1‑88 (MQ=255) aTGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAATGCCTGGCaa > 1:1666884/1‑90 (MQ=255) aTGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGGTTGCCCGTGAAAAACCCGGCaa > 1:1320840/1‑90 (MQ=255) ggCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAat < 2:661638/90‑1 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGCCTGGGAAattatt > 2:1229578/1‑90 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAATGGCTGGCAAattttt > 2:793455/1‑90 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGGCCGGGAAaatatt > 2:679309/1‑90 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGGCAAAAGCCTGGCAAattatt > 2:1591546/1‑90 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAattatt < 2:955594/90‑1 (MQ=255) tcgtATGGGCTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGAAAattatt < 2:371911/90‑1 (MQ=255) cgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGCCTGGCAAAATAttc > 2:721993/1‑90 (MQ=255) ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGTCTTCCCGTCAAAAGCCTGGGAAATTATTTTTtttc > 1:351006/1‑90 (MQ=255) ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTtctc < 1:296736/90‑1 (MQ=255) ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTtctc < 2:1617296/90‑1 (MQ=255) ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATAACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTtctc < 1:894474/90‑1 (MQ=255) gTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTCTCg > 2:1261113/1‑90 (MQ=255) ttCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGCAAAGCAAAAGCCTGGCAAAGTATTTTTTTTCgg > 1:1815061/1‑90 (MQ=255) ttCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAACCCGGCAAAATATTTTTTTTCgg > 1:1134958/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGCCCGTCAAAAGCCCGGCCAAATATTTTTTTTCGGc > 1:1567261/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 2:890671/90‑1 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 1:1261113/90‑1 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 1:976087/90‑1 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCaa > 2:141246/1‑90 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAGCCTTGGAAAATATTTTTTTTCCGCaa > 2:2177163/1‑90 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCaa < 1:1700823/90‑1 (MQ=255) cATGTTAGTTAATCAAAACGCCGTCGTTAATCCCCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAt < 1:852276/90‑1 (MQ=255) gttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGGAAATTATTTTTTTTCGGGAATgga > 1:1217636/1‑88 (MQ=255) gttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATgga > 2:372041/1‑88 (MQ=255) tagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTTCCCGTCAAAAACCCGGCAAATTATTTTTTTTCGGCAATGTACg > 1:1453899/1‑90 (MQ=255) tagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTACg > 2:1333493/1‑90 (MQ=255) gttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTTCCCGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTACGGa > 1:434311/1‑90 (MQ=255) ttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGAt < 2:945941/90‑1 (MQ=255) ttaATCAAAACGCCGTCGTTAATCACC‑ACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:2224609/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:459893/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:2239890/90‑1 (MQ=255) aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCAt < 2:2385378/90‑1 (MQ=255) aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCAt < 1:1333493/90‑1 (MQ=255) aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCAt < 1:785742/90‑1 (MQ=255) gCCGTCGTTAATCACCCACGGCGGGGAAGTCTGCCCGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 1:2507517/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 1:528497/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 2:1147140/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 1:118003/90‑1 (MQ=255) ccGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTACGGGTCCTCCTAAcccc > 2:528497/1‑89 (MQ=255) ccGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCa < 2:157504/90‑1 (MQ=255) gtcgtTAATCACCAACGGCGGGGGCGGCTGCCAGGCAAAAGCCTGGCAAATTATTTTTTTTCCGGCATGGACGGATCCTCATAACCCcga > 1:157504/1‑90 (MQ=255) cgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGGAAATTATTCTTTCTAGGCAATGTACGGATCCTCATAGCCCagaga < 1:171410/90‑2 (MQ=255) cgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc < 1:1522157/90‑1 (MQ=255) cgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc < 2:434311/90‑1 (MQ=255) cgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc < 1:1201045/90‑1 (MQ=255) cgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGAAATGTACGGATCCTCATAGCCCAGagc < 2:1178496/90‑1 (MQ=255) gtTAATCACCAACGGCGGGGGCGTCTGCCAGTCCAAAGCCTGGCAAAATATTCTTTTTCGGGAAAGTACGGGACCCCATAACCCCGagca > 1:1995376/1‑90 (MQ=255) ttAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaa < 2:1675558/90‑1 (MQ=255) ttAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaa < 2:653917/90‑1 (MQ=255) tCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCAt < 1:424145/90‑1 (MQ=255) cACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATa < 1:1591546/90‑1 (MQ=255) aCCAACGGCGGGGGCGGCTGCCCGTCAAAAGGCTGGCAAAATATTTTTTTTCGGCGAAGTACGGGTCCCCATAACCCCAAGCAAGCATaa > 1:2043959/1‑90 (MQ=255) aCCAACGGCGGGGACGTCTTCCCGTCAAAAGCCTGGCAAATTATTTTTTCTCGGCAATGTACGGATCCTCATAACCCAGAGCAAGCATaa > 2:1501305/1‑90 (MQ=255) aCCAACGGCGGGGACGGCTGCCAGTCAAAATCCTGGCAAATTATTCTTTTTCGGCAAAGTACGGATCCCCATACCCCAGAGCAAGCATaa > 2:2290789/1‑90 (MQ=255) aaCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtct < 1:2059500/90‑1 (MQ=255) caggcggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtctc < 2:1821953/88‑1 (MQ=255) aCGGCGGGGACGTCTGCCAGTCAAATGCCTAGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtcta < 1:1410384/90‑2 (MQ=255) gcggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtctcctg < 1:2034069/90‑4 (MQ=255) | TGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGT > NZ_CP009273/687219‑687386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |