Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	50,102	A→C	I94L (ATT→CTT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	50,102	0	A	C	100.0%	279.8 / NA	79	I94L (ATT→CTT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/45);  total (34/45)

CCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGAC  >  NZ_CP009273/50018‑50188                                                                                     |                                                                                       ccGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTggc                                                                                   >  2:394286/1‑90 (MQ=255)      tACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGcggcgg                                                                              >  1:1124018/1‑90 (MQ=255)          gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          >  2:1562237/1‑90 (MQ=255)          gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          <  2:1335349/90‑1 (MQ=255)          gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          >  2:1608257/1‑90 (MQ=255)          gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          >  2:2404306/1‑90 (MQ=255)          gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          <  1:1620012/90‑1 (MQ=255)             gATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGtt                                                                       >  2:1138315/1‑90 (MQ=255)              aTCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGttt                                                                      >  2:1373838/1‑90 (MQ=255)               tCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                                     <  1:2097829/90‑1 (MQ=255)               tCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                                     <  1:249536/90‑1 (MQ=255)               tCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                                     <  1:284362/90‑1 (MQ=255)                 gcgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATg                                                                   >  1:1707819/1‑90 (MQ=255)                 gcgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGATCGCGTTTATg                                                                   >  2:2134860/1‑90 (MQ=255)                  cgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          <  2:2190985/82‑1 (MQ=255)                  cgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          >  1:2190985/1‑82 (MQ=255)                  cgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGa                                                                  <  1:556968/90‑1 (MQ=255)                    tAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTggcgg                                                                                 >  2:1875516/1‑73 (MQ=255)                    tAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTggcgg                                                                                 <  1:1875516/73‑1 (MQ=255)                    tAACGTGGGTGAAGTCGGTGGATGAACCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                >  2:1402461/1‑90 (MQ=255)                        gTGGGTGAAGTCGGTGGGTGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGtt                                                            >  1:307079/1‑90 (MQ=255)                        gTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                                     <  1:1086984/81‑1 (MQ=255)                        gTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                                     >  2:1086984/1‑81 (MQ=255)                         tGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTc                                                           <  1:215616/90‑1 (MQ=255)                          gggTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCt                                                          >  2:1280794/1‑90 (MQ=255)                              gAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                <  2:1580458/80‑1 (MQ=255)                              gAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                <  2:2286972/80‑1 (MQ=255)                              gAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                >  1:2286972/1‑80 (MQ=255)                              gAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                >  1:1580458/1‑80 (MQ=255)                                  tCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCaaag                                                  <  1:973335/90‑2 (MQ=255)                                  tCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCaaaa                                                  <  1:6345/90‑1 (MQ=255)                                    ggTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                                     <  2:1237037/69‑1 (MQ=255)                                    ggTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                                     >  1:1237037/1‑69 (MQ=255)                                    ggTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAgc                                                >  1:1451747/1‑90 (MQ=255)                                        gATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaa                                            <  1:303660/90‑1 (MQ=255)                                        gATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaa                                            <  1:1566380/90‑1 (MQ=255)                                        gATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaa                                            <  1:507358/90‑1 (MQ=255)                                        gATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaa                                            <  1:1621655/90‑1 (MQ=255)                                         aTGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                                           <  1:1387871/90‑1 (MQ=255)                                         aTGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                                           >  2:51342/1‑90 (MQ=255)                                         aTGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                                           <  1:1379286/90‑1 (MQ=255)                                         aTGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                                           <  2:1188999/90‑1 (MQ=255)                                           gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaaa                                         >  2:67626/1‑90 (MQ=255)                                           gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaaa                                         <  2:1256535/90‑1 (MQ=255)                                            aaGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAAc                                        <  2:642360/90‑1 (MQ=255)                                                  tCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          >  1:1942329/1‑50 (MQ=255)                                                  tCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          <  2:1942329/50‑1 (MQ=255)                                                     cggcgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGa                               <  1:747983/90‑1 (MQ=255)                                                      ggcgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGAc                              <  1:1812540/90‑1 (MQ=255)                                                       gcgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGGCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACg                             >  1:2053615/1‑90 (MQ=255)                                                        cgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGc                            <  2:2014413/90‑1 (MQ=255)                                                         gtgtGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                <  2:907081/53‑1 (MQ=255)                                                         gtgtGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                >  1:907081/1‑53 (MQ=255)                                                           gtggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCata                         >  1:2453680/1‑90 (MQ=255)                                                             ggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATc                       <  2:771016/90‑1 (MQ=255)                                                             ggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATc                       <  1:1373838/90‑1 (MQ=255)                                                             ggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATc                       <  2:2053615/90‑1 (MQ=255)                                                             ggtgACGTACCAGAAATCATGGTGCTTGGCGGCGATCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATc                       <  1:2134860/90‑1 (MQ=255)                                                              gtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCg                      >  1:519303/1‑90 (MQ=255)                                                                 aCGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          <  2:1664018/35‑1 (MQ=21)                                                                 aCGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                          >  1:1664018/1‑35 (MQ=255)                                                                  cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGc                  <  2:807047/90‑1 (MQ=255)                                                                  cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGc                  <  1:1402461/90‑1 (MQ=255)                                                                  cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGAGCAAAAACTGTATCTGACGCATATCGACGc                  <  2:1934792/90‑1 (MQ=255)                                                                   gTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCa                 >  2:2433983/1‑90 (MQ=255)                                                                   gTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCa                 >  1:2266557/1‑90 (MQ=255)                                                                   gTACCAGAAAACATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCa                 >  1:1867924/1‑90 (MQ=255)                                                                    tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAg                <  2:1126858/90‑1 (MQ=255)                                                                        aGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                <  1:1340450/38‑1 (MQ=255)                                                                        aGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                >  2:2140941/1‑38 (MQ=21)                                                                        aGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                <  1:2140941/38‑1 (MQ=255)                                                                        aGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                                >  2:1340450/1‑38 (MQ=21)                                                                        aGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGt            <  2:1501676/90‑1 (MQ=255)                                                                          aaaTCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATc                                  <  2:477234/66‑1 (MQ=255)                                                                          aaaTCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATc                                  >  1:477234/1‑66 (MQ=255)                                                                           aaTCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCAAAAAGCGCAAAATCTGTATCTGACGCATATCGACGCAGAATTGGa         <  1:2404192/90‑1 (MQ=255)                                                                             tCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAg       <  2:1639583/90‑1 (MQ=255)                                                                              cATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGaggc      >  2:1821125/1‑87 (MQ=255)                                                                                  gTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGac  <  2:519303/90‑1 (MQ=255)                                                                                     |                                                                                       CCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGAC  >  NZ_CP009273/50018‑50188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑