| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,858,499 | G→C | W131C (TGG→TGC) | yeaD → | D‑hexose‑6‑phosphate mutarotase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,858,499 | 0 | G | C | 98.2% | 184.7 / ‑5.0 | 54 | W131C (TGG→TGC) | yeaD | D‑hexose‑6‑phosphate mutarotase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (22/31); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC > NZ_CP009273/1858414‑1858583 | ggACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGTCGAAAAAATTCTGCCCGc < 2:403626/90‑1 (MQ=255)ggACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGc < 2:99201/90‑1 (MQ=255) aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc < 2:580141/90‑1 (MQ=255) cTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGAc > 1:1385978/1‑90 (MQ=255) cTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGAc > 1:1875412/1‑90 (MQ=255) aTCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTAc < 2:1766488/90‑1 (MQ=255) aTCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTAc < 2:2293263/90‑1 (MQ=255) tCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACg < 2:2453559/90‑1 (MQ=255) cacaTCATGAAGATGCTGATGGCGTAGCACTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGc < 1:954794/90‑1 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGtt < 2:881970/90‑1 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGtt < 1:2041148/90‑1 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGtt < 1:28042/90‑1 (MQ=255) catGAAGATGCTTATAGCGTTGCGCTGACTTTTGAATTGACGCAAAGAGAAGAGCCGAAAAAATTCTGCCCGCACGACTTTACGCTGTTa > 1:2293263/1‑90 (MQ=255) catGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc < 1:51840/75‑1 (MQ=255) catGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc > 2:51840/1‑75 (MQ=255) catGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc > 2:907703/1‑75 (MQ=255) catGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc < 1:907703/75‑1 (MQ=255) tGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAgc > 1:345299/1‑90 (MQ=255) tGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAgc > 1:73688/1‑90 (MQ=255) aTGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAtt > 1:1190101/1‑90 (MQ=255) aTGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAtt > 2:181242/1‑90 (MQ=255) gCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑c > 2:140671/1‑90 (MQ=255) gCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATAT‑c > 1:1020439/1‑90 (MQ=255) gATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGGACGACTTTACGCTGTTAGCGCATTT‑Ccgc > 1:676754/1‑90 (MQ=255) aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑Ccgcg < 2:2190882/90‑1 (MQ=255) aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑Ccgcg > 2:721082/1‑90 (MQ=255) aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑Ccgcg < 2:1190101/90‑1 (MQ=255) aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑Ccgcg < 1:781210/90‑1 (MQ=255) tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGt < 1:870038/90‑1 (MQ=255) gCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACg > 2:307589/1‑68 (MQ=255) gCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACg < 1:307589/68‑1 (MQ=255) tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCGGGGCACACGACTATACGATGTTAGCGCAGTT‑GCGCGCggaaa > 1:2301047/1‑87 (MQ=255) gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCCGCGTGGGTAAAACtt > 2:943721/1‑90 (MQ=255) gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTtg > 2:917879/1‑90 (MQ=255) ttttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGGACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGa < 2:676754/90‑1 (MQ=255) tttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGaa > 1:2162462/1‑90 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATc < 2:2162462/90‑1 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATc < 2:388344/90‑1 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATc < 1:2448663/90‑1 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATc < 1:1439653/90‑1 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATc < 2:1533320/90‑1 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATc < 1:721082/90‑1 (MQ=255) aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATCg < 1:189789/90‑1 (MQ=255) aTTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATCGa < 2:2279903/90‑1 (MQ=255) gactcAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATCGATCt > 1:962378/5‑90 (MQ=255) gACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATCGATCt > 1:903669/1‑90 (MQ=255) gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGtt > 2:881986/1‑41 (MQ=255) gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGtt < 1:881986/41‑1 (MQ=255) gAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTaaaa > 1:1616556/1‑56 (MQ=255) gAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTaaaa < 2:1616556/56‑1 (MQ=255) aaTTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGt > 1:1848485/1‑43 (MQ=255) aaTTCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGt < 2:1848485/43‑1 (MQ=255) ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc < 2:1523053/90‑1 (MQ=255) ttCTGCCCGCACGACTTTACGCTGTTAGCGCATAT‑CCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc < 2:1020439/90‑1 (MQ=255) | GGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTT‑CCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC > NZ_CP009273/1858414‑1858583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |