| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,236,491 | A→C | F214C (TTT→TGT) | folE ← | GTP cyclohydrolase I FolE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,236,491 | 0 | A | C | 98.0% | 171.7 / ‑5.2 | 49 | F214C (TTT→TGT) | folE | GTP cyclohydrolase I FolE |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (23/25); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAC > NZ_CP009273/2236406‑2236575 | gCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTc > 2:1808209/1‑90 (MQ=255) aCGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTg < 2:613227/90‑1 (MQ=255) aCGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTg < 2:296111/90‑1 (MQ=255) gccgcGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGgc > 1:1227411/1‑90 (MQ=255) gccgcGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGgc > 1:1720758/1‑90 (MQ=255) cgcgAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGgcgc < 1:1527981/90‑1 (MQ=255) aaCAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTAt < 2:1379747/90‑1 (MQ=255) cGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGAtt > 2:1477364/1‑90 (MQ=255) tGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGAGCGTATTCTGACTGGATTTg > 2:1901337/1‑90 (MQ=255) cGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAAc < 1:850462/90‑1 (MQ=255) gTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACa < 2:1887817/90‑1 (MQ=255) tGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt < 2:1227411/90‑1 (MQ=255) tGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt < 1:1673797/90‑1 (MQ=255) tGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt < 2:813601/90‑1 (MQ=255) tGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt < 1:328838/90‑1 (MQ=255) gcTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATcca < 2:1195649/90‑1 (MQ=255) gcTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATcca < 2:1279510/90‑1 (MQ=255) ccATGGTTCCTGCCTTTTAATCAGTTGTGATTACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATccacca > 1:1841481/1‑90 (MQ=255) gTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACt > 2:1650737/1‑66 (MQ=255) gTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACt < 1:1650737/66‑1 (MQ=255) gTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACa < 1:1986094/77‑1 (MQ=255) gTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACa > 2:1986094/1‑77 (MQ=255) ccTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGt > 2:747257/1‑90 (MQ=255) ccTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGt > 2:2471347/1‑90 (MQ=255) tGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCg > 2:249175/1‑90 (MQ=255) cagcTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCg > 2:1171980/4‑90 (MQ=255) cagcTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCg > 2:1306783/4‑90 (MQ=255) gCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGt < 1:533798/90‑1 (MQ=255) gCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGt < 1:443637/90‑1 (MQ=255) ccTTTTAATCAGTTGTGATTACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGtt < 2:1841481/90‑1 (MQ=255) ttttAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGt > 1:2465741/1‑90 (MQ=255) tttAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTc > 1:495016/1‑90 (MQ=255) cAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCa < 1:2251581/90‑1 (MQ=255) cAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCa < 1:747257/90‑1 (MQ=255) gTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATcca > 1:1717123/1‑64 (MQ=255) gTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATcca < 2:1717123/64‑1 (MQ=255) tgtgATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAagag < 2:1657543/69‑1 (MQ=255) tgtgATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAagag < 2:2190000/69‑1 (MQ=255) tgtgATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAagag > 1:1657543/1‑69 (MQ=255) tgtgATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAagag > 1:2190000/1‑69 (MQ=255) gatgaCGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACt > 2:1649168/1‑85 (MQ=255) gatgaCGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACt < 1:1649168/85‑1 (MQ=255) caGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGa > 1:752224/1‑90 (MQ=255) gcgcgcAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATg > 2:23448/1‑90 (MQ=255) gcgcAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt < 2:2184204/43‑1 (MQ=255) gcgcAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt > 1:2184204/1‑43 (MQ=255) gcgcAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGcc > 1:2024123/1‑90 (MQ=255) gcgcAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGcc > 2:829624/1‑90 (MQ=255) gcAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAc < 1:2128856/90‑1 (MQ=255) | GCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAC > NZ_CP009273/2236406‑2236575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |