Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,171,725	G→C	D185H (GAC→CAC)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,171,725	0	G	C	100.0%	342.1 / NA	95	D185H (GAC→CAC)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (57/38);  total (57/38)

TTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCC  >  NZ_CP009273/4171642‑4171809                                                                                    |                                                                                      ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCg                                                                                 <  1:710467/90‑1 (MQ=255)        cGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGa                                                                          >  2:217568/1‑90 (MQ=255)         gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGAt                                                                         >  1:2043099/1‑90 (MQ=255)         gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGAt                                                                         >  1:686987/1‑90 (MQ=255)            aaaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCg                                                                      <  1:488345/90‑1 (MQ=255)             aaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGa                                                                     <  1:1137019/90‑1 (MQ=255)             aaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGa                                                                     <  1:2244801/90‑1 (MQ=255)                gTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTTGAATTCGATCCGAAgg                                                                  <  1:1332706/90‑1 (MQ=255)                 tAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGa                                                                 >  2:500570/1‑90 (MQ=255)                   aaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACa                                                               >  1:1916248/1‑90 (MQ=255)                      cccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc                                                            <  1:381074/90‑1 (MQ=255)                         aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCg                                                                      <  2:366153/77‑1 (MQ=255)                         aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCg                                                                      >  1:366153/1‑77 (MQ=255)                         aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGaa                                                                    >  2:177368/1‑79 (MQ=255)                         aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGaa                                                                    <  1:177368/79‑1 (MQ=255)                         aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                         >  1:2496133/1‑90 (MQ=255)                         aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                         <  2:2496133/90‑1 (MQ=255)                              tCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGTACAACCTGTTcgta                                                    >  2:2103662/1‑90 (MQ=255)                              tCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTcgta                                                    >  2:2007610/1‑90 (MQ=255)                                   tAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTAt                                              <  1:2007610/90‑1 (MQ=255)                                    aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATc                                             >  1:429495/1‑90 (MQ=255)                                      aGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGa                                           >  2:2395925/1‑90 (MQ=255)                                      aGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGa                                           >  1:1884271/1‑90 (MQ=255)                                          cTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc                                                            >  2:234133/1‑70 (MQ=255)                                          cTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc                                                            <  1:234133/70‑1 (MQ=255)                                          cTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGt                                       >  1:191704/1‑90 (MQ=255)                                          cTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGt                                       <  1:1854470/90‑1 (MQ=255)                                           tGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTc                                      >  1:939272/1‑90 (MQ=255)                                            gTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc                                                            >  1:1440770/1‑68 (MQ=255)                                            gTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc                                                            <  2:1440770/68‑1 (MQ=255)                                            gTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCg                                     <  2:2502783/90‑1 (MQ=255)                                                aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGt                                 <  2:1884271/90‑1 (MQ=255)                                                 actcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTa                                >  1:2032978/4‑90 (MQ=255)                                                 aCGCTCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGt                                       <  2:860504/83‑1 (MQ=255)                                                 aCGCTCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGt                                       >  1:860504/1‑83 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGAc                                                                >  1:2075049/1‑59 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGAc                                                                <  2:2075049/59‑1 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc                                                            <  1:2161751/63‑1 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAAcc                                                            >  2:2161751/1‑63 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTa                                >  2:1568129/1‑90 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTa                                >  2:394401/1‑90 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTa                                >  2:2084475/1‑90 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTa                                >  1:1698722/1‑90 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTa                                >  1:933137/1‑90 (MQ=255)                                                 aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTa                                >  2:900024/1‑90 (MQ=255)                                                   gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                         <  1:1769371/64‑1 (MQ=255)                                                   gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                         <  1:1047282/64‑1 (MQ=255)                                                   gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                         >  2:1047282/1‑64 (MQ=255)                                                   gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                         >  2:1769371/1‑64 (MQ=255)                                                   gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTaaa                              >  1:4825/1‑90 (MQ=255)                                                   gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTaaa                              >  1:2412189/1‑90 (MQ=255)                                                   gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTAGCCGTaaa                              >  1:1275106/1‑90 (MQ=255)                                                    cgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAAc                             <  2:497612/90‑1 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGa                                                                     <  1:1273459/49‑1 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGa                                                                     >  2:1273459/1‑49 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGTACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAAc                             >  2:89835/1‑88 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGTACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAAc                             <  1:89835/88‑1 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTc                                                       >  2:1242617/1‑63 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTc                                                       <  1:1242617/63‑1 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGt                                       <  2:346226/78‑1 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGt                                       >  1:346226/1‑78 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActg                           >  1:2495136/1‑90 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActg                           >  2:2480739/1‑90 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActg                           >  2:492580/1‑90 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActg                           >  1:1952549/1‑90 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActg                           >  2:1966025/1‑90 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActg                           >  2:1660850/1‑90 (MQ=255)                                                      cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActg                           >  2:161586/1‑90 (MQ=255)                                                      cgTATCATACCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATcc                                                                       >  1:2263165/1‑47 (MQ=255)                                                      cgTATCATACCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATcc                                                                       <  2:2263165/47‑1 (MQ=255)                                                         atcatcCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTAGCCGTAAActgcct                        <  2:1275106/90‑1 (MQ=255)                                                                cTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACca                 <  1:968386/90‑1 (MQ=255)                                                                cTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACca                 >  2:1762663/1‑90 (MQ=255)                                                                cTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACca                 <  1:500570/90‑1 (MQ=255)                                                                  tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGGTATCGACCGTCGCCGTAAACTGCCTGCGACcat                >  1:1954550/1‑90 (MQ=255)                                                                  tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACcatc               >  1:1872449/1‑90 (MQ=255)                                                                  tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACcatc               >  1:1649741/1‑90 (MQ=255)                                                                  tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACcatc               >  1:164887/1‑90 (MQ=255)                                                                  tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACcatc               >  1:1547398/1‑90 (MQ=255)                                                                    ccGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCg                                     <  2:895904/66‑1 (MQ=255)                                                                    ccGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCg                                     >  1:895904/1‑66 (MQ=255)                                                                      gTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGAc                                                                >  2:861991/1‑38 (MQ=255)                                                                      gTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGAc                                                                <  1:861991/38‑1 (MQ=255)                                                                      gTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTcgt                                                     <  2:1857391/49‑1 (MQ=255)                                                                      gTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTcgt                                                     >  1:1857391/1‑49 (MQ=255)                                                                      gTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTc           >  2:2355705/1‑90 (MQ=255)                                                                        ggTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAAc                             <  2:496774/70‑1 (MQ=255)                                                                        ggTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAAc                             >  1:496774/1‑70 (MQ=255)                                                                          ttCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcg       >  2:291281/1‑90 (MQ=255)                                                                          ttCCTGGATGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcg       <  1:30415/90‑1 (MQ=255)                                                                           tCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActgcct                        >  2:671131/1‑72 (MQ=255)                                                                           tCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAActgcct                        <  1:671131/72‑1 (MQ=255)                                                                           tCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc      <  2:2232586/90‑1 (MQ=255)                                                                           tCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc      <  1:1610812/90‑1 (MQ=255)                                                                           tCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc      <  2:191704/90‑1 (MQ=255)                                                                               ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  2:2412189/90‑1 (MQ=255)                                                                               ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  2:1952549/90‑1 (MQ=255)                                                                               ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  2:1739388/90‑1 (MQ=255)                                                                               ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  2:933137/90‑1 (MQ=255)                                                                               ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  2:939272/90‑1 (MQ=255)                                                                                    |                                                                                      TTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAC‑GTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCC  >  NZ_CP009273/4171642‑4171809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑