| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 409,689 | 0 | T | G | 53.8% | ‑2.3 / 11.7 | 13 | P507P (CCA→CCC) | sbcC | exonuclease subunit SbcC |
| Reads supporting (aligned to +/- strand): ref base T (6/0); new base G (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGG > NZ_CP009273/409614‑409777 | tAATGGAGACTGATAACCGCCGGGCCCCGGCGCCTGACCCCCCCCGCCCGCGGGGGGGGGGGGGAAACCAAAAGGGGGGCAAGGCTGAcc < 2:585407/90‑1 (MQ=255)tAACCGAGATGGATAAACGCCGGGCCCGGGCCCCGGATCCGGGTCGACCGCGGGGTGGTTGGGGGAACCAAAAAGGGGGCAAGGCTGAcc < 1:357455/90‑1 (MQ=255)tAAACGAGATTGATTACCGCCAGCCCCCGGCCCCTGATCCGCCCCGCCCGCGGGGGGGCGGGGGAAACCAAAAGGGGGGCAAGGCTGAcc < 2:75070/90‑1 (MQ=255) cTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTaa > 2:547316/1‑90 (MQ=255) tCCAGCGCCTGATCCGCCTCGACCGCCGGGTGGCTGGTGGACCCAAAAAGGGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCt < 1:104638/90‑1 (MQ=255) ccTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATgc > 1:526001/1‑90 (MQ=255) cGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATgcgcg > 2:15399/1‑90 (MQ=255) ggggggGTGGTGAAACCAAAAGGGGGGCAGGGCTGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGc < 2:440900/90‑1 (MQ=255) gggTGGCGGGTGGACCCAAAAGGGGGGAAGGGCTGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGc < 2:600911/90‑1 (MQ=255) ggggAACCACAATGGGGGCAGGGCCGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATg < 2:307692/88‑1 (MQ=255) aCCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGtttt > 2:372236/1‑90 (MQ=255) caAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTcaca > 2:339593/1‑90 (MQ=255) gTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCgg > 1:459566/1‑90 (MQ=255) | TAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGG > NZ_CP009273/409614‑409777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |