| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,254,473 | 0 | T | G | 53.3% | 10.1 / 16.9 | 15 | V76G (GTT→GGT) | ychH | stress‑induced protein YchH |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTG > NZ_CP009273/1254386‑1254562 | tGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGGGCGGCCCGTACTGGGGGGGTc > 2:144456/1‑90 (MQ=255) aaGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGGTCGCCa > 1:226539/1‑90 (MQ=255) aaGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGTGCGACCGTTACTGGTGGGGTCGccc > 1:356245/1‑89 (MQ=255) aaGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGGGGTGGGGGGGATGAAAAGGGGGGGGGCCCTTACTGGGGGGGTTGCCa > 2:443353/1‑90 (MQ=255) ttttCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGTGTTGGCGGGGATGAAAAGGGGTGCGACCCTTTCTGGGGGGGTTGCCACTATg > 2:483855/1‑90 (MQ=255) ttttCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGTGTTGGCGGGCATGAACAGGGGTGCGACCGTTACTGGGGGGGTCGCCCCTATg > 1:352773/1‑90 (MQ=255) ttttCGTCGGTGCCATTCTCTGGCTGGCGGGGGCCCGGGTTGGCGGGCATGAAAAGGGGTGCGACCGTTAATGGGGGGGTCGCCACTATg > 2:28686/1‑90 (MQ=255) cgGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGGGTGCGACCGTTACTGGGGGGGTCGCCCCTATGACAAACg > 2:71950/1‑90 (MQ=255) cGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACa < 1:497621/90‑1 (MQ=255) cAGGTGTGCGCCCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAg < 2:421338/90‑1 (MQ=255) cAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAg < 1:443353/90‑1 (MQ=255) gCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTg < 2:24363/90‑1 (MQ=255) gTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACcagt > 2:108541/1‑90 (MQ=255) gTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACcagt < 2:356245/90‑1 (MQ=255) ggTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTgcgc < 1:182105/90‑1 (MQ=255) ttCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTg < 1:71950/90‑1 (MQ=255) | TGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCGCCGTAGCGATAATCGCCGTCATAGCTAACAAATAATGCAGTTTGCTGACCAGTCAGTTTGCGCTG > NZ_CP009273/1254386‑1254562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |