Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,331,2020AC54.5% 6.8 / 10.8 12G566G (GGT→GGG) gyrADNA topoisomerase (ATP‑hydrolyzing) subunit A
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCA  >  NZ_CP009273/2331121‑2331278
                                                                                 |                                                                            
gaAGCAAAGAATGGGGTCGTGGGTGTTCCCCCCCAGCAGTCGGTCGAAAAATTCTTTTTTTTTAATACGTGCGGCAGTtttccctttccc                                                                      <  2:192638/90‑1 (MQ=255)
        gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCacgac                                                              >  1:532703/1‑90 (MQ=255)
              ggTCGTGAGTGTCCCCCACCAGCAGTCGGTCGAAAAAGTCTTTTTCTTTAATACGGGGGGAAGTTTTCCCTTCCCCGCCACGACGCTgcg                                                        <  2:567847/90‑1 (MQ=255)
                  gTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTc                                                    >  1:246490/1‑90 (MQ=255)
                  gTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTc                                                    >  2:404153/1‑90 (MQ=255)
                                ccaGCAGGCGGCAGAAAAAGTTTTTTTTTTTAATACGTGCGGCAGATTTTCCTTTCCCGCCACGTCGCTGCGCTTCGTATTCAGAAAGCg                                      <  1:358751/90‑1 (MQ=255)
                                    cagTCGGTCGAAAAAGTCTTCTTTTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTg                                  <  1:179098/90‑1 (MQ=255)
                                                   gTCTTTTTCTTTAATCCGTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGcc                   <  1:404153/90‑1 (MQ=255)
                                                   gTCTTCTTTTTTAATCGGTGCGGCAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGcc                   <  2:84117/90‑1 (MQ=255)
                                                   gTCTTCTTTTTAAATCCGGGCGGCAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGcc                   <  2:246490/90‑1 (MQ=255)
                                                             ttAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagag         >  1:526213/1‑90 (MQ=255)
                                                             ttAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagag         >  2:436439/1‑90 (MQ=255)
                                                                    gggCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa  <  2:532703/88‑1 (MQ=255)
                                                                                 |                                                                            
GAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCA  >  NZ_CP009273/2331121‑2331278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑