Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,587,9740AC53.8% 8.7 / 13.0 13G415G (GGT→GGG) tmcAtRNA cytosine(34) acetyltransferase TmcA
Reads supporting (aligned to +/- strand):  ref base A (5/1);  new base C (0/7);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCG  >  NZ_CP009273/2587909‑2588060
                                                                 |                                                                                      
accaGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGcc                                                                >  1:118817/1‑90 (MQ=255)
             gcgGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGt                                                   >  2:577146/1‑90 (MQ=255)
                 gCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCCCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTc                                               <  2:214304/90‑1 (MQ=255)
                           agtGCCCATACCGCCTGACAGAGTTGTTGAGAAAACCCCCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAgccgcc                                     <  2:535407/88‑1 (MQ=255)
                              gCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAgc                                                                   >  1:479225/1‑57 (MQ=255)
                              gCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAgc                                                                   <  2:479225/57‑1 (MQ=255)
                                       gCCTGACTGAGTTGTTGAGATAATCCCCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGt                         <  1:226644/90‑1 (MQ=255)
                                       gCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAAtct                         >  1:535407/1‑88 (MQ=255)
                                        ccTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGtt                        >  1:67976/1‑90 (MQ=255)
                                            actgaGTTGTTGAGATAATCCCCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGccc                    <  1:561773/90‑1 (MQ=255)
                                             ctgaGTTGTTGAGTTAATCCCCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCt                   <  1:343391/90‑1 (MQ=255)
                                                   tgttgAGATAACCCCCCCTCACCCCCCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGca             <  2:254270/90‑1 (MQ=255)
                                                              tCCCCCCTCATCCCCCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCg  <  1:294252/90‑1 (MQ=255)
                                                                 |                                                                                      
ACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCG  >  NZ_CP009273/2587909‑2588060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑