| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,477 | 0 | G | A | 56.2% | ‑0.9 / 13.9 | 16 | E676K (GAA→AAA) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base G (6/1); new base A (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTG > NZ_CP009273/2852398‑2852566 | tATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCaa > 2:92963/1‑90 (MQ=255)tATCGACCGCCTCTTCCCCCGCGTGGGCGCGGCAAATGCCCGGGCGCCCGGGCACCAAACCTTTAGGGGGGAGTTGACTGAAACCGCCaa < 1:247000/90‑1 (MQ=255) tcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATAtttt > 2:10054/1‑90 (MQ=255) tCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATa > 1:521632/1‑90 (MQ=255) tccccGCGAGGGCGGGGCAGATGCCCGGGCGCCGGGGCGCTCAACTTTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 1:287198/88‑1 (MQ=255) ggCAGATGCCCGGGCGCCGGGGCCCCCAACTTTTAGGGGGGGAATGACGAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 2:551963/90‑1 (MQ=255) gCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTg > 2:178984/1‑90 (MQ=255) gATGCCCGGGCGCCCGGGCCCCCACCTTTTGTGGGGGAGATGACAAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGgtg < 2:295804/90‑1 (MQ=255) cccgggCGCCGGGGCGCTCACCTTTTAGGGGGGAGATGACAAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTaa < 2:220183/86‑1 (MQ=255) cccTGGCGCCCGGGCGCCAAACTTTTGGGGTGGAGAGGATTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTaa < 2:477918/89‑1 (MQ=255) cgcccGGGCGCTCAACTTTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATg < 1:289626/87‑1 (MQ=255) cGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATg < 1:10054/90‑1 (MQ=255) gggCGCTAACCTTTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 1:59170/90‑1 (MQ=255) cttttATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGaa < 1:308775/88‑1 (MQ=255) aTGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTcc > 1:605535/1‑90 (MQ=255) ggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTa > 1:79390/1‑90 (MQ=255) gAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTg > 2:601236/1‑90 (MQ=255) | TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTG > NZ_CP009273/2852398‑2852566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |