| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,126,661 | 0 | T | G | 56.2% | 9.2 / 16.7 | 16 | H219P (CAC→CCC) | yghS | dTMP kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAAC > NZ_CP009273/3126577‑3126735 | aaaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGc < 2:60589/90‑1 (MQ=255) aaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGcc < 1:471576/90‑1 (MQ=255) tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATcc > 1:83850/1‑90 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCCTCCCGTTCa > 2:517837/1‑90 (MQ=255) cATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAgg < 2:239564/90‑1 (MQ=255) aTTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCTAGGa > 2:514537/1‑90 (MQ=255) ttttGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGGCCCTCCCGTTCAAGGag > 1:333464/1‑89 (MQ=255) ttttGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAAGAt > 1:76272/1‑90 (MQ=255) ttttGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCCCCCGGTCAAgggg > 2:81844/1‑88 (MQ=255) tGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCtt < 1:517837/90‑1 (MQ=255) aTCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcgc < 2:365103/90‑1 (MQ=255) aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCAttta < 2:471365/90‑3 (MQ=255) gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGATCTTTTCGCCCTTGAATg > 1:390818/1‑90 (MQ=255) cggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCCCCCCCTTCAAAGAACTTTTTGCCCTTTAATGtta > 2:515626/1‑88 (MQ=255) gcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCAAGGGTCTTTGCGCCCTTTAATGTCAGt > 1:394629/1‑90 (MQ=255) aTTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAAc < 1:7513/90‑1 (MQ=255) | AAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAAC > NZ_CP009273/3126577‑3126735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |