| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,363,673 | 0 | G | T | 64.3% | ‑3.4 / 12.3 | 14 | L13I (CTT→ATT) | nanK | N‑acetylmannosamine kinase |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base T (0/9); total (3/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATG > NZ_CP009273/3363588‑3363751 | aaGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTa > 1:47286/1‑90 (MQ=255) ggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGc > 2:375093/1‑90 (MQ=255) gcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGAAATTTTAGTACCGCCGATATCAATCGCCAgtg < 1:376701/90‑1 (MQ=255) gTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGtgtg < 1:436598/90‑1 (MQ=255) gcgGATCTCCCCGTCACCGCCAATCGGCGGGGCGGAAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTc < 2:558195/90‑1 (MQ=255) tctcCCCTTCAGCGCCAATCGCCGGGGGGGCAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGc < 2:81619/90‑1 (MQ=255) tctcCCCGCCAGCCCCAATCAGCGGGGGGGAAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGc < 2:47286/90‑1 (MQ=255) tCTGCCCGTCAGCGCAAATCGGCGGGGCGGAAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGc < 1:417261/90‑1 (MQ=255) ctccccGTCAGCCCCAACCAGCGGGGCGGCATTTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCt < 2:554324/87‑1 (MQ=255) cTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCt < 1:375093/90‑1 (MQ=255) cTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTCCATCGCt > 2:11568/1‑90 (MQ=255) aGCGCCAATCGGCGGGGCGCCAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTAc < 2:567453/90‑1 (MQ=255) ccccAATCAGCGCGGCGGAAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCa < 2:587337/88‑1 (MQ=255) cgcgGGGGAAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAAtg < 1:225738/90‑1 (MQ=255) | AAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATG > NZ_CP009273/3363588‑3363751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |