| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,594,161 | 0 | C | T | 52.6% | 3.7 / 41.5 | 38 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (1/17); new base T (20/0); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-06 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGG > NZ_CP009273/4594077‑4594240 | aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttcttttt < 2:195624/90‑1 (MQ=255) cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttctttttc < 1:177432/90‑1 (MQ=255) tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGcc < 1:142475/90‑1 (MQ=255) cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATgaga > 1:11318/1‑90 (MQ=255) cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCGTTTTTTTTTTTTTTTCCCTgaga > 2:487579/1‑90 (MQ=255) cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTTTTTTTTTTCCCTgaga > 2:409516/1‑90 (MQ=255) cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTTTTTTTTTGCCATgaga > 2:76971/1‑90 (MQ=255) tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTTTTTTTTTGCCATgagga > 2:462299/1‑88 (MQ=255) cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGt < 2:508756/90‑1 (MQ=255) tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc < 2:163321/90‑1 (MQ=255) ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAgcgga > 2:181612/1‑88 (MQ=255) ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCa > 2:608431/1‑90 (MQ=255) ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCa > 2:286735/1‑90 (MQ=255) taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag < 1:487579/90‑1 (MQ=255) ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag < 1:290511/90‑1 (MQ=255) cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga < 2:61235/90‑1 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGGTCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCagagag > 1:456671/1‑90 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGGCTTTTTTTTTTTTTTTGCC‑TGGGAAGGGAGCGCagggag > 2:441166/1‑90 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCGTTTTTTTTTTTTTTGCCCTGAGAAGAGAGCGCagagag > 2:417203/1‑90 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCagagag > 2:177129/1‑90 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAgg > 2:82521/1‑90 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGGGAAGTGAGCGCAGAGAgg > 1:560354/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAggg > 1:108267/1‑90 (MQ=255) cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa < 2:100981/90‑1 (MQ=255) cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa < 1:286735/90‑1 (MQ=255) ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAgggggt > 2:111376/1‑87 (MQ=255) cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGAGAGGGGTTAtttt > 1:115931/1‑90 (MQ=255) cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCtt > 1:135510/1‑90 (MQ=255) aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATTAGAAGTGGGGGCAGAGGGGGGTTATCttt > 2:494038/1‑89 (MQ=255) tttGAGGATGCTAAGTCGCGGATCTCGTTTGCGGC‑GGTCGTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAGCTTGc < 2:475722/90‑1 (MQ=255) tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc < 2:92589/90‑1 (MQ=255) tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc < 2:241050/90‑1 (MQ=255) tCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGGTTTTCTTTCCTGACTGAAATGGCg > 1:447826/1‑90 (MQ=255) tcGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGttt < 1:292880/90‑1 (MQ=255) gcggc‑gGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGt > 1:545880/1‑90 (MQ=255) gc‑ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc < 2:11318/90‑1 (MQ=255) c‑ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc < 2:195839/90‑1 (MQ=255) ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg < 1:186743/90‑1 (MQ=255) ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg < 2:71267/90‑1 (MQ=255) gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg < 1:152685/90‑1 (MQ=255) | ACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGG > NZ_CP009273/4594077‑4594240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |