Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	50,102	A→C	I94L (ATT→CTT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	50,102	0	A	C	100.0%	94.5 / NA	29	I94L (ATT→CTT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/11);  total (18/11)

GGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAA  >  NZ_CP009273/50020‑50182                                                                                   |                                                                                 gggTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTggcgg                                                                           >  1:182245/1‑90 (MQ=255)    tACGGACGATCGCGTAACGTGGGTGGAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGcggcgg                                                                        >  2:134862/1‑90 (MQ=255)        gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                    <  1:479731/90‑1 (MQ=255)           gATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGtt                                                                 >  2:368385/1‑90 (MQ=255)           gATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGtt                                                                 >  1:186712/1‑90 (MQ=255)           gATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGtt                                                                 >  2:531810/1‑90 (MQ=255)             tCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                                               >  1:105620/1‑90 (MQ=255)                  tAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc                                                          <  2:77714/90‑1 (MQ=255)                        gggTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                    >  2:580902/1‑74 (MQ=255)                        gggTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc                                                                    <  1:580902/74‑1 (MQ=255)                        gggTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGGTTATGAACAGTTCt                                                    >  2:297336/1‑90 (MQ=255)                         ggTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCtt                                                   >  2:86685/1‑90 (MQ=255)                          gTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTg                                                  >  2:335225/1‑90 (MQ=255)                                 cGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAg                                           >  2:454795/1‑90 (MQ=255)                                   gTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAgcg                                         >  1:578090/1‑90 (MQ=255)                                      gATGAAGCCATCGCGGCGTGTGGTTACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaa                                      <  2:497815/90‑1 (MQ=255)                                       aTGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                                     <  1:439922/90‑1 (MQ=255)                                        tGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaa                                    >  2:445816/1‑90 (MQ=255)                                          aaGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAAc                                  <  1:106358/90‑1 (MQ=255)                                            gCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTg                                <  2:491834/90‑1 (MQ=255)                                               aTCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTAt                             >  2:123522/1‑90 (MQ=255)                                               aTCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTAt                             >  2:605119/1‑90 (MQ=255)                                                    ggcgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGAc                        <  2:290723/90‑1 (MQ=255)                                                      cgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGc                      <  1:543633/90‑1 (MQ=255)                                                         gtggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCata                   >  2:546334/1‑90 (MQ=255)                                                           ggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATc                 <  2:186712/90‑1 (MQ=255)                                                                  tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAg          >  1:348040/1‑90 (MQ=255)                                                                    ccAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGaa        >  1:116988/1‑90 (MQ=255)                                                                          aTCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGaa  <  1:86685/90‑1 (MQ=255)                                                                                   |                                                                                 GGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAA  >  NZ_CP009273/50020‑50182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑