| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,858,499 | G→C | W131C (TGG→TGC) | yeaD → | D‑hexose‑6‑phosphate mutarotase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,858,499 | 0 | G | C | 100.0% | 52.3 / NA | 17 | W131C (TGG→TGC) | yeaD | D‑hexose‑6‑phosphate mutarotase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (7/10); total (7/10) | |||||||||||
ACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC > NZ_CP009273/1858416‑1858583 | aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc < 2:472915/90‑1 (MQ=255)aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc < 2:372966/90‑1 (MQ=255)aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc < 1:84508/90‑1 (MQ=255) cTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGAc > 2:482196/1‑90 (MQ=255) aTCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTAc < 1:305955/90‑1 (MQ=255) tGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAttt > 2:353291/1‑90 (MQ=255) tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc < 2:519865/64‑1 (MQ=255) tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc > 1:519865/1‑64 (MQ=255) aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg < 1:107499/90‑1 (MQ=255) cTTTTGAATTGACGCAAAGCGAAGAAACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGGAAAACTtgtg > 1:370365/1‑90 (MQ=255) ttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt < 1:439755/73‑1 (MQ=255) ttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt > 2:439755/1‑73 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATc < 1:87536/90‑1 (MQ=255) gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATc < 2:251450/90‑1 (MQ=255) gACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt > 1:554816/1‑90 (MQ=255) gACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt > 1:307382/1‑90 (MQ=255) ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc < 2:224366/90‑1 (MQ=255) | ACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC > NZ_CP009273/1858416‑1858583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |