| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,236,491 | A→C | F214C (TTT→TGT) | folE ← | GTP cyclohydrolase I FolE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,236,491 | 0 | A | C | 100.0% | 39.2 / NA | 13 | F214C (TTT→TGT) | folE | GTP cyclohydrolase I FolE |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (8/5); total (8/5) | |||||||||||
TGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGAT > NZ_CP009273/2236404‑2236570 | tGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGacac > 1:168740/1‑90 (MQ=255) gCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTc > 1:159540/1‑90 (MQ=255) gccgcGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGgc > 2:402273/1‑90 (MQ=255) cAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTc < 1:575054/90‑1 (MQ=255) tCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACt < 1:16708/90‑1 (MQ=255) gAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTgg > 1:128674/1‑90 (MQ=255) tGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt < 1:402273/90‑1 (MQ=255) tGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt < 2:133545/90‑1 (MQ=255) tGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCg > 1:140388/1‑90 (MQ=255) tGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCg > 1:568619/1‑90 (MQ=255) ttAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCg < 1:81013/90‑1 (MQ=255) gTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACt > 2:207553/1‑90 (MQ=255) acaGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCgg > 1:84190/1‑90 (MQ=255) aGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGAt > 2:566641/1‑90 (MQ=255) | TGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGAT > NZ_CP009273/2236404‑2236570 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |