| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,370,587 | G→T | R65S (CGT→AGT) | sspA ← | stringent starvation protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,370,587 | 0 | G | T | 94.7% | 58.3 / NA | 19 | R65S (CGT→AGT) | sspA | stringent starvation protein A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (6/12); minor base A (1/0); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGA > NZ_CP009273/3370505‑3370673 | tAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATccacc < 1:80843/90‑1 (MQ=255) aaaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATccacca < 2:437419/90‑1 (MQ=255) aaaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATccacca < 2:296578/90‑1 (MQ=255) cATCAGTGGCGGATGCGGGAAAAGCACATCCAGAAATTCCATAATGATGCGAGATTCCCACCGGGTCCGCTCACACTTCACCAGGTTCgg > 2:332543/1‑90 (MQ=255) tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGTCGGaa < 1:543436/90‑1 (MQ=255) gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGt > 2:482828/1‑79 (MQ=255) gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGt < 1:482828/79‑1 (MQ=255) cGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGTCGGAACGctc < 2:554998/82‑1 (MQ=255) cGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGTCGGAACGctc > 1:554998/1‑82 (MQ=255) cTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTc < 1:584035/90‑1 (MQ=255) ttCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTg > 1:589048/1‑90 (MQ=255) aTAATGATGCGAGATTCCCACAGGGTCAGCTCACTATccacca > 1:207200/1‑43 (MQ=255) aTAATGATGCGAGATTCCCACAGGGTCAGCTCACTATccacca < 2:207200/43‑1 (MQ=255) gatgCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt < 1:332543/90‑1 (MQ=255) gCGAGATTCCCACAGGGTCAGCTCACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTc < 1:164392/90‑1 (MQ=255) gggTCAGCTCACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt > 1:66728/1‑90 (MQ=255) cAGCTCACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAt > 2:425588/1‑68 (MQ=255) cAGCTCACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAt < 1:425588/68‑1 (MQ=255) cACTATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGa < 2:204702/90‑1 (MQ=255) | TAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGA > NZ_CP009273/3370505‑3370673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |