Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,171,725	G→C	D185H (GAC→CAC)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,171,725	0	G	C	100.0%	73.7 / NA	23	D185H (GAC→CAC)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/9);  total (14/9)

TTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCC  >  NZ_CP009273/4171641‑4171809                                                                                     |                                                                                     tttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTc                                                                                 <  1:605128/90‑1 (MQ=255)             aaaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCg                                                                     <  2:441917/90‑1 (MQ=255)                     aaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACaa                                                             <  1:331764/90‑1 (MQ=255)                         cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTg                                                         >  1:331360/1‑90 (MQ=255)                          aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                        >  2:57937/1‑90 (MQ=255)                          aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGt                                                        >  2:441500/1‑90 (MQ=255)                                       aGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGa                                           >  2:287296/1‑90 (MQ=255)                                           cTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGt                                       <  2:137886/90‑1 (MQ=255)                                                  aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTa                                >  1:125207/1‑90 (MQ=255)                                                  aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTa                                >  2:230704/1‑90 (MQ=255)                                                  aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTa                                >  2:385017/1‑90 (MQ=255)                                                    gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATAGACCGTCGCCGTaaa                              >  2:551817/1‑90 (MQ=255)                                                       cgTATCATCCCTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAActg                           >  2:248756/1‑90 (MQ=255)                                                               cccTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGAc                   <  1:230704/90‑1 (MQ=255)                                                               cccTTACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGAc                   <  2:440230/90‑1 (MQ=255)                                                                   tACCGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACcatc               >  1:163093/1‑90 (MQ=255)                                                                   tACCGTGGTCCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTaaa                              >  2:352939/1‑75 (MQ=255)                                                                   tACCGTGGTCCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTaaa                              <  1:352939/75‑1 (MQ=255)                                                                      cGTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCAtt            >  2:592096/1‑90 (MQ=255)                                                                       gTGGTTCCTGGCTGCACTTCGAATTCGATCCGAAGGCCAACCTGCTCGCACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCCTTc           >  2:281533/1‑90 (MQ=255)                                                                         ggTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCg                     <  1:483979/78‑1 (MQ=255)                                                                         ggTTCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCg                     >  2:483979/1‑78 (MQ=255)                                                                            tCCTGGCTGCACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATAGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc      <  1:551817/90‑1 (MQ=255)                                                                                ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  2:377782/90‑1 (MQ=255)                                                                                ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  2:192325/90‑1 (MQ=255)                                                                                ggctgcACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc  <  1:385017/90‑1 (MQ=255)                                                                                     |                                                                                     TTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCC  >  NZ_CP009273/4171641‑4171809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑