| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 497,028 | 0 | A | C | 51.8% | 27.1 / 38.4 | 27 | V556G (GTG→GGG) | ybaL | Kef family K(+) transporter |
| Reads supporting (aligned to +/- strand): ref base A (13/0); new base C (0/14); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACC > NZ_CP009273/496960‑497113 | gcgggCGCGTTTTACCATGCCTACTTTTTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCg < 1:1782877/87‑1 (MQ=255)gCTGGCGCTTCTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCg < 2:304923/90‑1 (MQ=255) cgcgTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTcc > 1:1011956/1‑90 (MQ=255) ttCTTATCATCCCTAATTTTTTTTCATATTTTCCCCCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc < 2:1525506/89‑1 (MQ=255) gTCTTATCATGCCTCCATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc < 2:375585/90‑1 (MQ=255) gTCTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc < 1:674670/90‑1 (MQ=255) acagtATCATGCCTACTTTTTTTTCATTTTTTCCACCGGACACCCCCCTTTCCCCCCCCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc < 2:1192526/86‑1 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 1:464418/1‑90 (MQ=255) ccTACATTTTTTTCATTTTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:988004/90‑1 (MQ=255) ccTACATTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:1307759/90‑1 (MQ=255) ccTACATTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:1455019/90‑1 (MQ=255) ccTACATTTTTTTAATTTTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:1442509/90‑1 (MQ=255) aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCactgt > 1:1192526/1‑86 (MQ=255) aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt > 1:1579783/1‑90 (MQ=255) ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc > 2:1696743/1‑90 (MQ=255) tatTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc > 2:5294/1‑90 (MQ=255) acccGCCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc < 1:1684581/88‑1 (MQ=255) cccGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 1:801346/89‑1 (MQ=255) ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt > 2:1248649/1‑90 (MQ=255) ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt > 1:463174/1‑90 (MQ=255) cccGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc < 1:1350276/89‑1 (MQ=255) gTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGa > 2:1871866/1‑90 (MQ=255) ttcccccGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt < 1:1267663/87‑1 (MQ=255) ttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt > 2:1674203/1‑90 (MQ=255) ccGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcac > 1:988127/1‑90 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc > 2:1499153/1‑90 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc > 2:1750669/1‑90 (MQ=255) | GCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACC > NZ_CP009273/496960‑497113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |