| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,318 | 0 | G | A | 57.6% | ‑2.7 / 32.4 | 33 | intergenic (‑124/+79) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (12/2); new base A (0/19); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-06 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGA > NZ_CP009273/1819232‑1819392 | aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa > 1:982661/1‑90 (MQ=255)aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa > 1:514842/1‑90 (MQ=255) cgggggATGTTTATCGGCAACGGGGGTGATTTACGTTGGGGGTGATCGGGTTTTTTTTACAATTGTTTGAGAAGAAAAAAAAAAAccgcc < 2:1129318/86‑1 (MQ=255) ccGTGGTTGTTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAAccgcc < 2:1971/90‑1 (MQ=255) ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc > 2:901687/1‑90 (MQ=255) ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc > 2:1194683/1‑90 (MQ=255) gttGGTTATCGGCAACTGGGGTATTTTACGTTGGGGGTGATCAGGTTTTTTTTACACCTTTTGGAGAAAAAAAAAAAAAACCGCCGATcc < 1:1314706/88‑1 (MQ=255) ggTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGt > 2:191957/1‑90 (MQ=255) ttATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTcc < 1:1389678/90‑1 (MQ=255) ttATCGGCAACTCGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAGAAAAAAAAACCGCCGATCCTGTcc < 1:368010/90‑1 (MQ=255) accGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCAc < 1:31944/88‑1 (MQ=255) tCGGCAACTGGGGTGTTTTATTTTGGGGGTGACCGGTTTTTTTTTACACCTTTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCAcc < 2:1720342/90‑1 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 2:1765440/1‑90 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 1:1720342/1‑90 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 2:574549/1‑90 (MQ=255) gggTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGc < 1:1128714/90‑1 (MQ=255) gtgtTTTACGTTAGTGGTGATCAGGTTTTTTTTACACCTGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:901687/90‑1 (MQ=255) gTGATTTATGTTAGTGGTCATCAGTTTTTTTTTACATTTTTTTGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 2:982661/90‑1 (MQ=255) gTGATTTACTTTAGTGGTGAGCGGTTTTTTTTTACAATTTTTGGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 2:1572345/90‑1 (MQ=255) gTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:966481/90‑1 (MQ=255) gTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:332982/90‑1 (MQ=255) ttACTTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:1194683/90‑1 (MQ=255) ttACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:563235/90‑1 (MQ=255) ttACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg > 2:555214/1‑90 (MQ=255) gTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGa > 1:45629/1‑90 (MQ=255) ggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAc > 1:322601/1‑90 (MQ=255) gATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 1:199768/1‑90 (MQ=255) aTCGGGTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:698189/90‑1 (MQ=255) aTCGGGTTTTTTTAAAAATTGTTAAAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:895391/90‑1 (MQ=255) aTCAGTTTTTTTTTACAACTTTTTAAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:1815104/90‑1 (MQ=255) gCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt > 1:1627052/1‑90 (MQ=255) ttttttACATTTTTTGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTaa < 2:432904/90‑1 (MQ=255) ttttttACAATTTTTGGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTaa < 2:54699/90‑1 (MQ=255) gTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTgggtt > 1:1046092/1‑87 (MQ=255) tttGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGa < 1:1257849/90‑1 (MQ=255) | AGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGA > NZ_CP009273/1819232‑1819392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |