Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,090,8260TG68.6% 4.1 / 18.8 35G67G (GGT→GGG) hemWradical SAM family heme chaperone HemW
Reads supporting (aligned to +/- strand):  ref base T (2/9);  new base G (24/0);  total (26/9)
Fisher's exact test for biased strand distribution p-value = 7.79e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-07
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTA  >  NZ_CP009273/3090737‑3090914
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gaTTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCggtggt                                                                                          <  1:1006221/90‑1 (MQ=255)
 aTTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCgggggtg                                                                                         >  1:240075/1‑89 (MQ=255)
          aGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGcc                                                                                <  2:1306945/90‑1 (MQ=255)
            cATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTg                                                                              <  1:1755532/90‑1 (MQ=255)
              tCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGAAGCCGGGCCTGCt                                                                            >  2:1773419/1‑90 (MQ=255)
              tCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACCCCGAGCCTGCt                                                                            >  2:1491204/1‑90 (MQ=255)
                   ttAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCttttcc                                                                       >  2:629582/1‑87 (MQ=255)
                   ttAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGAACCCGAGCCCGCTTTccg                                                                       >  2:763603/1‑90 (MQ=255)
                    tAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGGGCCCGCGTTtcgg                                                                      >  1:441291/1‑90 (MQ=255)
                          tCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCCGGCCTGCCTTccggcccggc                                                                >  2:761966/1‑90 (MQ=255)
                           cTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGGCGCCCAGCCCGCGTTCCGGGCCcgcg                                                               >  1:1573101/1‑90 (MQ=255)
                                  aCGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGAGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCaaa                                                        >  2:503527/1‑90 (MQ=255)
                                   cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAAc                                                       <  1:761966/90‑1 (MQ=255)
                                   cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTACGGCCCGGCGATGCAAAc                                                       <  2:992288/90‑1 (MQ=255)
                                   cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACCCCCAGCCCTCCTTCCTGGCCGGGGAGGGAAAc                                                       >  2:63112/1‑90 (MQ=255)
                                     aTGTGGCTTCCGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACgc                                                     <  2:522915/90‑1 (MQ=255)
                                       gtgGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGAACCCCAGCCCGCCTTTCGGCCCGGGGATGGAAACgctc                                                   >  1:856096/1‑89 (MQ=255)
                                             tACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTCCTGGAc                                             <  2:1115473/90‑1 (MQ=255)
                                             caggCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACTCTGCTGGAc                                             <  2:626222/87‑1 (MQ=255)
                                                        ccGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTTCCGCCCGGCGGTGCAAAAGCTGCTGGACGGCgggcgggc                                  >  2:1713962/1‑90 (MQ=255)
                                                        ccGTGAAGTAAAGACAATCTTTATTGGCGGGGGGCGCCCCGGCCCGCTTTTCCGCCCGGCGCCGCCAACGCCGTTGGTGGgggtgcgggt                                  >  2:1166453/1‑87 (MQ=255)
                                                        ccGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGAGGCCGCTTTTCCGCCCCGCGATGCAAAAACTGCTGGGCggcgggcgggg                                  >  1:1706022/1‑87 (MQ=255)
                                                         cGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGGGCCCGCTTTCCGGCCCCGCGATGGAAAAGCTGCTGGGCggcgggcgggcg                                 >  1:799044/1‑90 (MQ=255)
                                                          gTGAAGTAAAGACAATCTTTATTGGCGGTGGGGCGCCGGGCCTGCTTTTCGGCCCGGCGGAGCAAAAACTGCTGGACGGCGggcgtgcgg                                >  1:33509/1‑89 (MQ=255)
                                                          gTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGCGCCCGCTTTTCCGCCCGGGGGGGCAAAACCTGCTGGGAGGCGGGGGTgggc                                >  1:1786436/1‑90 (MQ=255)
                                                               gTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCGGCGGTGCAAACGCTGCTGGGCGGGGGGCGGGGGCGgttt                           >  2:1357697/1‑89 (MQ=255)
                                                               gTAAAGACAATCTTTATTGGCGGTGGGACGCCCAGCCTGCTTTCCGGCCCGGCGGTGCAAACGCTGCTGGACGGGGTGCGTGCGCGGTTg                           >  2:245126/1‑90 (MQ=255)
                                                               gTAAAGACAATCTTTATTGGCGGTGGGACGCCCAGCCCGCTTTTCCGCCCCGCGATGCAAACGCTGCTGGACGGCGTGCGGGGGCGtttt                           >  1:1540348/1‑89 (MQ=255)
                                                               gTAAAGACAATCTTTATTGGCGGGGGGACGCCCAGCCCGCTTTCCGGCCCGGCGGTGCAAACGCTGCTGGGCGGGGGGCGGGCGCGGTTg                           >  2:1297654/1‑90 (MQ=255)
                                                                tAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGATGCTGGACGGCGTGCGTGCGCGTTTGc                          <  1:1660029/90‑1 (MQ=255)
                                                                       aaTCTTTATTGGCGGTGGGGCGCCGAGCCTTCTTTTCGGCCCCGCGGTGCAAAAGCTGCTGGGCGGGGGGGGGGCGCGGTTGGCGCCggg                   >  1:1276974/1‑89 (MQ=255)
                                                                        aTCTTTATTGGCGGTGGGACGCCGGGCCTGCTTTCCGGCCCGGCGATGCAAAAACCGCTGGGCGGGGGGCGTGGGGGGTTTGCGCTGGcc                  >  1:507020/1‑89 (MQ=255)
                                                                         tCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCCGCGATGCAAACACTGCTTTACGGCGGGCGGGCGCGGTTTCCGCTGGGAg                 >  2:872976/1‑90 (MQ=255)
                                                                         tCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCCGCGATGCAAAAGCTGCTGGGCGGCGGGCGGGCGCGGTTTCCCCTGGCAg                 >  2:1213319/1‑90 (MQ=255)
                                                                                  gCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAg        <  1:1627590/90‑1 (MQ=255)
                                                                                   cGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGGTGCAGa       >  1:1309144/1‑90 (MQ=255)
                                                                                   cGGTGGGACGCCGAGCCCGCTTTCCGGCCCGGCGATGCAAAACCTGCTGGGCGGGGGGGGGGCGCGGTTGCCGCCGGGCGCGGGTGCCGa       >  1:1330265/1‑90 (MQ=255)
                                                                                       ggtACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAAtt   <  2:33509/90‑1 (MQ=255)
                                                                                       ggtACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAAtt   <  2:456108/90‑1 (MQ=255)
                                                                                       ggtACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAAtt   <  2:1540349/90‑1 (MQ=255)
                                                                                       ggtACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAAtt   <  2:886442/90‑1 (MQ=255)
                                                                                        gtACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTa  <  2:934711/90‑1 (MQ=255)
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GATTATGTTCAGCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTA  >  NZ_CP009273/3090737‑3090914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑