Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,594,1670CT50.0% 48.5 / 58.1 74S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
Reads supporting (aligned to +/- strand):  ref base C (6/31);  new base T (37/0);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 6.98e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGG  >  NZ_CP009273/4594078‑4594256
                                                                                         |                                                                                         
cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                           <  2:1835815/90‑1 (MQ=255)
  cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                         <  1:1522485/90‑1 (MQ=255)
    tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGcc                                                                                       >  2:613277/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTgagg                                                                                 >  1:214458/1‑89 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTgaga                                                                                 >  2:270655/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATaaga                                                                                 >  2:785045/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATgaga                                                                                 >  2:684323/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTCTTTTTTTTCCATgtga                                                                                 >  1:1596054/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTgaga                                                                                 >  1:168011/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCCTTCTGTTTGTAATTTTTTTCCATgagg                                                                                 >  2:299619/1‑89 (MQ=255)
           tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                <  1:684323/90‑1 (MQ=255)
            ccATACCTATACCAGTCAGGAGTCATTTCAGGATTCCATGTCGCGGATCTCGTTTGCGGCGGTCCATTTTTTTTTTTTTTCCCTGGGAAg                                                                               >  2:966381/1‑90 (MQ=255)
            ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAg                                                                               >  2:110/1‑90 (MQ=255)
             cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                              <  1:190260/90‑1 (MQ=255)
             cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGAGGCCATTTTTTCTTTTTCTGCCATGAGAAga                                                                              >  2:1407754/1‑89 (MQ=255)
              aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTg                                                                             <  1:152667/90‑1 (MQ=255)
                 ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                          <  1:1418578/90‑1 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGTCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCATGAGAAGGGAgggc                                                                        >  2:77927/1‑90 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAgcgc                                                                        >  2:720005/1‑90 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAgcgc                                                                        >  2:412024/1‑90 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                        <  1:1710946/90‑1 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                        <  1:481886/90‑1 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                        <  1:1488004/90‑1 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                        <  2:1742900/90‑1 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                        <  1:785045/90‑1 (MQ=255)
                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTTAGAAGGGAgcgga                                                                       >  1:173272/1‑88 (MQ=255)
                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTTGAAGTGAGCGCa                                                                       >  1:1879410/1‑90 (MQ=255)
                      aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCaga                                                                     >  2:1920860/1‑90 (MQ=255)
                       ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                    <  1:626534/90‑1 (MQ=255)
                       ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                    <  1:840245/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  1:979597/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  1:429653/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  1:138655/90‑1 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTCCATTTGAAATGAGCGGagagag                                                                  >  1:1336585/1‑90 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGGGCagagag                                                                  >  2:1718748/1‑90 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCagagag                                                                  >  2:275922/1‑90 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGGGAAGGGAGCGCcgggag                                                                  >  2:837962/1‑90 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCcgggag                                                                  >  2:1418417/1‑90 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTg                                                                                         >  1:803917/1‑67 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                         <  2:803918/67‑1 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                                  <  1:270655/90‑1 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGAGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaggg                                                                  >  1:1855633/1‑88 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTCCCTGAGAAGAGAGCGCCGGGAgg                                                                 >  2:416445/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGCGAAATGGGCGGAGAGAgg                                                                 >  2:429653/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATGAGAAGTGGGCGCAGAGAgg                                                                 >  1:1819122/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCagggaggg                                                                >  1:892854/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAggg                                                                >  2:658047/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGGGGGCGCAGAGAggg                                                                >  2:1201632/1‑90 (MQ=255)
                            cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGTGGGCGCAGCGggggg                                                               >  2:1067489/1‑89 (MQ=255)
                                cagcATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                           <  1:1072414/87‑1 (MQ=255)
                                   cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCtt                                                        >  2:417671/1‑90 (MQ=255)
                                        cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctg                                                   <  2:200488/90‑1 (MQ=255)
                                         aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGGGGGGGGGTATTTTGCctga                                                  >  1:894567/1‑90 (MQ=255)
                                           gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGGGGGGGGTTTTCTTTCcttact                                                >  1:267043/1‑90 (MQ=255)
                                               ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAAc                                            >  2:1332909/1‑90 (MQ=255)
                                                cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                           <  1:1718747/90‑1 (MQ=255)
                                                cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                           <  2:214458/90‑1 (MQ=255)
                                                cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                           <  1:359291/90‑1 (MQ=255)
                                                  tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGGGAGCGCAGGGGGGGGGTATCTTGCCTGACTGAACTgg                                         >  1:312471/1‑90 (MQ=255)
                                                  tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGGGGGCAGAGAGGGGGTATTTTGCCTGACTGaaattg                                         >  2:690245/1‑86 (MQ=255)
                                                    tCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCGGAGAGGGGGTATCTTTCCTGACTGAACTGGCg                                       >  1:1555130/1‑90 (MQ=255)
                                                    tCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGGGGGGGGTTATTTTGCCTGACTGAACTGGCg                                       >  1:908037/1‑90 (MQ=255)
                                                            ctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                               <  1:1067488/90‑1 (MQ=255)
                                                            ctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                               <  2:908038/90‑1 (MQ=255)
                                                               gTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGATATCTTTCCTTACTGAACTGGCGATTAAGttttc                            >  1:654370/1‑88 (MQ=255)
                                                                   gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGt                        <  1:110/90‑1 (MQ=255)
                                                                    cggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTa                       <  2:654370/90‑1 (MQ=255)
                                                                     ggcggTCATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGGAc                      >  2:724708/1‑90 (MQ=255)
                                                                      gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                     <  1:417671/90‑1 (MQ=255)
                                                                       cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                    <  2:449609/90‑1 (MQ=255)
                                                                       cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                    <  2:1596055/90‑1 (MQ=255)
                                                                        gggcATTTTTTCTTTTTCTGCCCTGAGGAGGGAGCGCAGAGGGGGATTATCTTGCCTGACTGAACTGGGGATTAAATTTCCCCGTACCCg                   >  2:1409758/4‑90 (MQ=255)
                                                                        ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg                   <  1:136307/90‑1 (MQ=255)
                                                                         gTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg                  >  1:1719884/1‑90 (MQ=255)
                                                                            aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt               <  2:215594/90‑1 (MQ=255)
                                                                                tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg           <  1:366113/90‑1 (MQ=255)
                                                                                 ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGt          <  1:210958/90‑1 (MQ=255)
                                                                                 ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGt          <  1:498376/90‑1 (MQ=255)
                                                                                       ttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc    <  1:982105/90‑1 (MQ=255)
                                                                                         ctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg  <  1:1920859/90‑1 (MQ=255)
                                                                                         ctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg  <  2:1487334/90‑1 (MQ=255)
                                                                                         ctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg  <  2:312471/90‑1 (MQ=255)
                                                                                         |                                                                                         
CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGG  >  NZ_CP009273/4594078‑4594256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑