| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 409,689 | 0 | T | G | 50.0% | ‑4.1 / 11.5 | 18 | P507P (CCA→CCC) | sbcC | exonuclease subunit SbcC |
| Reads supporting (aligned to +/- strand): ref base T (8/1); new base G (0/9); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTC > NZ_CP009273/409614‑409757 | tAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGAcc < 1:28730/90‑1 (MQ=255) gagcCTGATAACCGCCCGCCCCCCGCGCCTGATCCGCCACGCCCGCGGGGGGGGGGGGGAACCCAAAAAGGGGGAAAGGCTGACCCGCCt < 1:1914454/90‑1 (MQ=255) cTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTaa > 2:857643/1‑90 (MQ=255) cTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTaa > 2:1380912/1‑90 (MQ=255) cTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTaa > 2:1874891/1‑90 (MQ=255) gATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGTCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACt > 1:986073/1‑90 (MQ=255) cccAGCCCCCGGCCCCTGATCCCCCCCCCCCGCCGGGTGGCGGGGGAACCCACAAGGGGGGCAAGGCTGCCCCGCCTGTAACTGTGCACg < 1:1802635/89‑1 (MQ=255) cAGGCTCCAGCGCCTGATCCGCCTCGCCCGCCGGGTGGCTGGTGGAACCACAAAGGGGGCAAGGCTGACCCGCCTGTAACTGTGCACGtt < 2:323554/90‑1 (MQ=255) aGGCTCCAGCGCCTGATACGCCTCGACCTCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTg > 2:390382/1‑90 (MQ=255) aGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTg > 1:517463/1‑90 (MQ=255) cAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTc > 1:1533941/1‑90 (MQ=255) ccccTCGCCCGCGGGGTGGCGGGGGGAACCAAAAAGGGGGAAAGGCGGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGAt < 2:1205208/88‑1 (MQ=255) cTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATgcg > 2:358689/1‑90 (MQ=255) tCGCCCGCGGGGTGGCTGGTGGACCAAAAAGGGGGGCAGGGCTGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATgcgc < 1:342481/90‑1 (MQ=255) ccgcccgcggGGTGGGGGGGGAACCCAAAAGGGGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATgcgc < 1:1380911/86‑1 (MQ=255) ccgcccgcggGGTGGCTGGGGGACCCAAAAGGGGGGAAGGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATgcgc < 2:727600/86‑1 (MQ=255) ggggggCGGGTGGACCCAAAAGGGGGCCAAGGCTAGCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGc < 2:988101/90‑1 (MQ=255) gtgggtggGGGAACCAAAAAGGGGGCAAGGCTGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTc < 2:660150/90‑1 (MQ=255) | TAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTC > NZ_CP009273/409614‑409757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |