| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,963 | 0 | T | G | 47.4% | 9.5 / 29.0 | 38 | D492A (GAC→GCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/19); new base G (18/0); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTA > NZ_CP009273/822881‑823050 | aaaGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGcc < 1:1250790/90‑1 (MQ=255) ggtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGGAACGCCGGGAGTggg > 2:677993/1‑90 (MQ=255) cATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACCCCGGAAGTCGGgtcgtc > 2:1151798/1‑90 (MQ=255) cACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGACGGAAGTCGGTTCGTCgag < 2:54477/90‑1 (MQ=255) cACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGACGGAAGTCGGTTCGTCgag < 2:210423/90‑1 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAACCCGGAAGTCGGGTCGTCGAGAAAc > 1:583871/1‑90 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACCCCCGAAGGCGGGTCGTCGGGaaaa > 2:613300/1‑89 (MQ=255) tGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACa < 1:1266528/90‑1 (MQ=255) tGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACa < 1:374700/90‑1 (MQ=255) gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAGGCGGGTTGTCGGGAAAAAg > 1:1811641/1‑90 (MQ=255) gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCCGAAGTCGGGTTGTCGAGAAAAAg > 2:1189456/1‑90 (MQ=255) agtctcTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTc < 2:131363/88‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 2:1867045/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 1:685978/90‑1 (MQ=255) tACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGGGAAACAGAAAGTCCGGGTc > 2:506954/1‑90 (MQ=255) cATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGCAAGCCGGGAGGCGGGTCGGCGGGAAAAAGAAAGACCGGGTCGTg > 1:291613/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGTGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:319005/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 1:1903764/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:233861/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTAGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 1:832850/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGTGGGGCAACGCCGGGAGTCGGGTGGTCGAGAAAAAGAAAGTCCGGGTCAttc > 1:319005/1‑88 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGTCGGGTCGTCGAGAAAAAAAAAATCCGGGTCATGc > 2:685978/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGAAGGGGGGTCGTCGGGAAAAAAAAAGTCCGGGTCAttc > 2:1136371/1‑88 (MQ=255) tGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtc < 1:413663/61‑1 (MQ=255) tGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTtc > 2:413663/1‑61 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGGCGGGGgcatgcat > 1:1602637/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGGCGGGTCGTCGGGAAAAAGAATGTCCGGGTGATTCATc > 1:233861/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg < 1:174716/90‑1 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGGGAAAAAAAAAGGCCGGGTCATGCCTCAg > 1:902902/1‑90 (MQ=255) tGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCg > 1:1262057/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAGACGGTTCGTCGGGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAgg > 1:1621513/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTCGGGTCGTCGGGAAAAAGAAAGGCCGGGTCATGCATCAGCGAACAGgcc > 2:174716/1‑90 (MQ=255) aTTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgccagcgcca < 1:506954/90‑1 (MQ=255) ggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCt < 2:20162/90‑1 (MQ=255) gTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTa < 1:1189455/90‑1 (MQ=255) gggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCt > 2:1810114/1‑90 (MQ=255) ggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTa < 2:1811642/90‑1 (MQ=255) ggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTa < 1:156833/90‑1 (MQ=255) | AAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTA > NZ_CP009273/822881‑823050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |