| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,313 | 0 | G | A | 46.4% | 17.0 / 17.1 | 28 | intergenic (‑119/+84) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (13/2); new base A (0/13); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.83e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAG > NZ_CP009273/1819224‑1819395 | tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑gagaagag < 2:1104040/90‑1 (MQ=255) aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaagaaa > 2:627587/1‑90 (MQ=255) ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAgaaaagaaaa > 2:1454300/1‑90 (MQ=255) aCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAAccgc > 2:196347/1‑90 (MQ=255) cggTGGATGTTTATCGCAACCGGGGGTATTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAAAAAAAAAAAAAAAAccgcc < 1:1454299/88‑1 (MQ=255) ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAAccgcc > 1:145314/1‑90 (MQ=255) ggggATGTTTATCGGCAACTCGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTT‑GAGAAGAGAAAAAAAAACCGCCGa < 2:696516/88‑1 (MQ=255) gATGTTTATCGGAAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATcc < 2:680315/90‑1 (MQ=255) ttACCGCCACCTGGGGTGATTTACGTTTGTGGTGATCGGGTTTTTTTTAAAATTTTTT‑GAGAAGAAAAAAAAAACCCGCCGATCCTGTcc < 1:196347/90‑1 (MQ=255) aaCTCGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 2:1158425/90‑1 (MQ=255) aCTCGGGTGATTTACGATAGTGGGGATCAGGGTTTTTTTACAACTGTTT‑GAGAAGGGAAAAGAAAACCGCTGATCATGTTCACCGCagga > 1:533192/1‑87 (MQ=255) gtgtTTTACGTTGGGGGTGATCAGGTTTTTTTTACAACTGTTT‑GAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 2:1886741/90‑1 (MQ=255) gTGATTTACTTTAGTGGTGATCAGTTTTTTTTTAAAACTGTTG‑GAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 1:1612435/90‑1 (MQ=255) aggATTTACGTTGGTGGTGATCAGTTTTTTTTTACACCTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:1330396/88‑1 (MQ=255) ttACGTTAGTGGTGATCGGTTTTTTTTTACAATTTTTT‑GAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:711193/90‑1 (MQ=255) tccGTTGGTGGTGACCAGTTTTTTTTTAAAACTTTTTAGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:877491/88‑1 (MQ=255) aGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAcct > 2:1330397/1‑88 (MQ=255) gtggtgATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg > 1:938649/1‑90 (MQ=255) gtggtgATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg > 1:296741/1‑90 (MQ=255) gATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 1:959418/1‑90 (MQ=255) gATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 1:1269778/1‑90 (MQ=255) aTCGGGTTTTTTTTAAACCTGTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:396624/90‑1 (MQ=255) aTCAGTTTTTTTTTACAATTGTTG‑AAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 1:1623216/90‑1 (MQ=255) cAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg > 1:1277687/1‑90 (MQ=255) ttttttA‑AACTTTTTGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGATCTTaa < 1:1474279/90‑1 (MQ=255) ttttt‑GAGAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt < 1:1249067/88‑1 (MQ=255) tGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg > 2:1633255/1‑60 (MQ=255) tGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg < 1:1633254/60‑1 (MQ=255) gTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTTTTAAGTTTTTTGGTTg > 1:365757/1‑90 (MQ=255) gTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg > 2:1715817/1‑90 (MQ=255) gTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTg > 2:202572/1‑90 (MQ=255) gaaaagagaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg < 2:61741/87‑1 (MQ=255) | TATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAG > NZ_CP009273/1819224‑1819395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |