| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,687,654 | 0 | T | C | 54.5% | 10.0 / 20.2 | 22 | I417V (ATC→GTC) | purL | phosphoribosylformylglycinamidine synthase |
| Reads supporting (aligned to +/- strand): ref base T (7/3); new base C (0/12); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGT > NZ_CP009273/2687570‑2687740 | tCATTGCGGGGCCGCGAAAAACCCCCAGCTCCGCGCCGCCGTTAACCCCGCCTTTTTGTACGTTATCGGCGCGAATGTTGCCGACcccgc < 2:1899604/90‑1 (MQ=255)tCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATcccgc > 2:200531/1‑90 (MQ=255) cggggCCGCAGAGAACCACCAGGTTCGCCCCGGGGTTGCCCTCCCCTTTTTGTACGTGATCGGCGGGATTGTTGCCGACCCCGCCCGCCa < 1:764041/88‑1 (MQ=255) ccGGGCCGCCGAGAACGGCCAGCTCCCCCCGGTCTTTGCCCTCTCTTTTTTGTCCGTGATCGGGGCGAATGTTGCCGACCCCCCCCGCCa < 1:1481769/90‑1 (MQ=255) ccgAGAACGCCCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATc < 2:1634209/90‑1 (MQ=255) gCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAAc > 1:910423/1‑90 (MQ=255) gCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGACCCCGCCCGCCAGCATGATCGGTTTGTGATAAc < 2:588449/90‑1 (MQ=255) gccccGGCGTTGATCTCGCTTTTTTGTAGGTGCTGGGCGGGAATGTTGCCGCCCCCGCCCGCCAGCATGATCGGTTTGTGATAACCgcgc < 2:930863/87‑1 (MQ=255) gACGTTGATCACGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGctc > 2:93356/1‑90 (MQ=255) gACGTGGTTCTCGCCTTTTTGTACGTGTTCGGCGCGAATGTTCCCGATCCCCCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGctc < 2:1323760/90‑1 (MQ=255) gTTGCTCCCCCCTTTTTGTCCGTGCTGGGCGAAAATGTGCCCGACCCCCCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTc < 1:93356/90‑1 (MQ=255) ttGATCTCCCTTTTTTGTACGTGATCGGCGCAAATTTTGCCGACCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCg < 2:648365/90‑1 (MQ=255) tcGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTtg > 1:176783/1‑90 (MQ=255) cGCTTTTTTGTACGTGATCGGCGCGAATGTTGCCGACCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTtgt < 2:1164600/90‑1 (MQ=255) cGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTtgt > 1:669843/1‑90 (MQ=255) cGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTtgt > 1:1493168/1‑90 (MQ=255) cTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGc < 2:1460904/90‑1 (MQ=255) ttttGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTg > 2:157264/1‑90 (MQ=255) tttGTAGGTGATCGGCGCGAATGTTGCCGACCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt < 2:185233/90‑1 (MQ=255) cGTGCTCGGCGCAATTTTTGCCGCCCCCCCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACtt < 1:972938/90‑1 (MQ=255) gcgcGATTGTTGCGGACCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCa < 2:311069/90‑1 (MQ=255) cGACCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGt < 1:1242770/90‑1 (MQ=255) | TCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGT > NZ_CP009273/2687570‑2687740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |