| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,774,215 | 0 | T | G | 52.9% | 11.3 / 16.3 | 17 | G343G (GGT→GGG) | lldD | quinone‑dependent L‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGAT > NZ_CP009273/3774137‑3774286 | tATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCt > 2:53122/1‑90 (MQ=255) gcgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGgt < 2:604091/72‑1 (MQ=255) gcgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGgt > 1:604091/1‑72 (MQ=255) gcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCCGCTAAATCt > 2:216752/1‑90 (MQ=255) cTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTg < 2:557525/90‑1 (MQ=255) cGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGAt > 1:336030/1‑90 (MQ=255) cGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGAt > 1:507369/1‑90 (MQ=255) cGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAAACTGCTAAATCTGAt > 2:49377/1‑90 (MQ=255) ggTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATc < 2:221932/90‑1 (MQ=255) gACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGaaaaa < 1:111716/90‑1 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGAt > 1:549369/1‑90 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCTGCTAAAACTGATCGAAAAAGAGAt > 2:12628/1‑90 (MQ=255) gctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGt > 2:18355/1‑90 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGa < 1:12628/90‑1 (MQ=255) ttGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACt < 2:449593/90‑1 (MQ=255) tGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGgcgc < 2:458318/90‑1 (MQ=255) cgcTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAATGGCGATGACGCTGACTGGGGCGa > 1:450491/1‑90 (MQ=255) tGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGGGGGGATGACGCTGACTGGCGCGAAAt > 2:89643/1‑90 (MQ=255) aaCAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGAt > 1:77939/1‑90 (MQ=255) | TATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGAT > NZ_CP009273/3774137‑3774286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |