| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 40,515 | 0 | G | T | 53.3% | 12.7 / 20.6 | 15 | Q473K (CAA→AAA) | caiT | L‑carnitine/gamma‑butyrobetaine antiporter |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base T (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGAT‑AATGCCAACCAGAATTGACCAACCGATACGCAC > NZ_CP009273/40434‑40590 | gTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGGTTTAATTgggt > 1:98511/1‑88 (MQ=255) gcgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGGTTTGAGTGGGttttgg > 2:228190/1‑87 (MQ=255) tCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGGTTTGATCGGGTTTAGggcg > 2:110390/1‑90 (MQ=255) ggAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTTAATCGGGTTTAGGCCGCCGAGCGcccg > 1:131572/1‑88 (MQ=255) gagaGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCcagc < 2:405841/90‑1 (MQ=255) gagaGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCcagc < 2:85744/90‑1 (MQ=255) cGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAAc < 2:399581/90‑1 (MQ=255) gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTTAATCGGTTTTAGGCCGCCGGGCGCCCGCAGAAAAAAAACGAT‑aaaggc > 1:179997/1‑86 (MQ=255) gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTTAATCGGTTTTAGGCCGCCGAGCGCCAGCAGAAAAATACCGAT‑AATGcc > 1:6211/1‑90 (MQ=255) gCGGGCATCCTCCGGCGATAATGGC‑GGTTTTAAATGGGTTTAGGCCGCCGCGCGCCCGCAGAAAAAAAACCATAAAACCCAACCAAAAtt > 2:106053/1‑90 (MQ=255) ggCATCCTCCGGCGATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGAT‑AATGCCAACCAGAATTGAcc < 1:158067/90‑1 (MQ=255) cATCCTCCGGCGATAATGGC‑GGTTTTAATCGGTTTCAGGCCGCCGAGCGCCCGCAGAACAATACCGAT‑AATGCCAACCAGAATTGACCaa > 1:11234/1‑90 (MQ=255) cATCCTCCGGCGATAATGGC‑GGTTTTAATCGGGTTTAGGCCGCCGGGCGCCCGCAGAAAAAAAACCAA‑AAATCCAACCAGAATTGACCaa > 1:245425/1‑90 (MQ=255) cGATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGAT‑AATGCCAACCAGAATTGACCAACCGATACGCa < 1:38029/90‑1 (MQ=255) gATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGAT‑AATGCCAACCAGAATTGACCAACCGATACGCaa < 2:376787/90‑2 (MQ=255) | GTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTGAATCGGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCGAT‑AATGCCAACCAGAATTGACCAACCGATACGCAC > NZ_CP009273/40434‑40590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |