Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,776,2680TG55.0% 8.2 / 16.6 21T252P (ACC→CCC) gpsANAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base G (11/0);  total (14/7)
Fisher's exact test for biased strand distribution p-value = 2.17e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG  >  NZ_CP009273/3776180‑3776355
                                                                                        |                                                                                       
ccAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTa                                                                                        <  2:264722/90‑1 (MQ=255)
             gcgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGGAAACACCAAAACGc                                                                           >  1:20621/1‑90 (MQ=255)
             gcgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGGAAACACCAAAACGc                                                                           >  1:397914/1‑90 (MQ=255)
                        aCATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGcc                                                                <  2:266832/90‑1 (MQ=255)
                           tCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATg                                                             <  1:389914/90‑1 (MQ=255)
                            ccATGCCCTGACCTAGCATCATGCCAAAACGGCGGGTTCCCGGCCGGGTGGCGGGTAAGGGAAACACCCGAAACCCAAGCcccccccagc                                                            >  2:220182/1‑86 (MQ=255)
                            ccATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCACCGCCGGGTGTTGGTACAAGGAAACACCACATCCCCCAGCCCCCCCACGc                                                            >  2:221097/1‑90 (MQ=255)
                            ccATGCCCTGACCGAGCATCATGCCAAAACGGCGGGTACGCGGCTGGTTGTCGGGACAGGGAAGGACCAAAACGCCAAACCCCGCCATGc                                                            >  1:410875/1‑90 (MQ=255)
                            ccATGCCCTGACCGACCATCATGCCAAAACGGGGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGc                                                            <  1:221097/90‑1 (MQ=255)
                              aTGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGTAAGCACCAAAACGCCAAACCCCCCCCTGccc                                                          >  1:407538/1‑90 (MQ=255)
                                gCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTTGGGACAGGGAAGCACCAAAACGCCAAACCCCCCCCAGcccca                                                        >  1:207955/1‑88 (MQ=255)
                                   cTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATaaa                                                     <  2:44964/90‑1 (MQ=255)
                                     gACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAAATCGCCAAGCCCCGCCATGCCCATAAAgg                                                   >  1:161892/1‑90 (MQ=255)
                                     gACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAAATCGCCAAACCCCCCCATGCCCCTAAAgg                                                   >  1:99130/1‑90 (MQ=255)
                                     gACCGAGCATCATGCCAAAACGGCGGTTACGCGACCGGTTGTCGGTGCAGGGAAGCACCAAAACGCCCAACCCCCCCCCGCCCCTaaaag                                                   >  2:389965/1‑88 (MQ=255)
                                     gACCGAGCATCATGCCAAAACGGCGGTTAAGCGACTGGGTGTCGGGACAGGGAAACACCAAATCGCCAAGCCCCCCCCTGCCCCTaaaag                                                   >  1:294913/1‑88 (MQ=255)
                                                 tccaaaaaCGGCGGTTACGCGCCTGGTTGTCGGTACAGGAAAGCACCAGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCgg                                       <  2:294913/86‑1 (MQ=255)
                                                           gcggTTACGCGACTGGTTGTCGGGACAGGGAAACACCAAAACGCCAAACCCCCCCCTGCCCCTAAAGGGGGGAGGGGCGGCACCCAgcgc                             >  2:99558/1‑90 (MQ=255)
                                                                          gTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGa              <  1:79724/90‑1 (MQ=255)
                                                                                    cAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagcc    >  1:305150/1‑90 (MQ=255)
                                                                                      ggTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagccag  >  2:54595/1‑90 (MQ=255)
                                                                                        |                                                                                       
CCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG  >  NZ_CP009273/3776180‑3776355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑