| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,368,611 | 0 | G | A | 53.8% | 3.4 / 12.2 | 13 | Q51* (CAG→TAG) | ampC | BlaEC family class C beta‑lactamase |
| Reads supporting (aligned to +/- strand): ref base G (6/0); new base A (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAG > NZ_CP009273/4368535‑4368700 | aacaacGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgcc > 2:135009/1‑90 (MQ=255) gtgtgAGGGGTTGTTTTTGGGCGTTGCCCGAATACCCCCAGGAAAAGTAATAAGTTTTCCCCTAAAAAATTCCCGCCACCGCCATACCCg < 1:29536/90‑1 (MQ=255) gcGGGCTGTTTTTGGGGGATGCCCCCATCCCCCAAGGAAAAGAAAAAAGTTTTACCCTAAAAAATTCCCCCCCCCGCCATACCCGGGATc < 1:337720/89‑1 (MQ=255) cGGGCTGTTTTTTGGCGATGCCCGCATACCCCCAGGAAAAGTAAAAAGTTTTCCCCTAAAAAATTCCCGCCACCGCCATACCCGGGATCt < 1:154766/90‑1 (MQ=255) ttttGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGct > 2:153117/1‑90 (MQ=255) aTGCCCGCATAGCCCCGGGAAAAGTAATAGGTTTTCCCTTAATAATTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc < 1:229990/90‑1 (MQ=255) ctCCGCATAGCCCAAGGAAAATAAATAAGTTTTCCCCTAAAAATTCCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCgg < 1:403679/89‑1 (MQ=255) aaaaaGAAATAGGTTTTCCCCTAAAAAATCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGc < 2:379223/88‑1 (MQ=255) aGTAATAAGTTTTCCCTTAAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACaa < 1:343627/90‑1 (MQ=255) ataaGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATc > 1:323382/1‑90 (MQ=255) gTTTACCCTGATAAATTACCGCCCCCGCCATACCCGGGATTTTTTGTTGCTCTATAAGCGGGGAAATTGTGCGAGGCACAATATCGTTgt > 2:151127/1‑89 (MQ=255) ttACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt > 1:12065/1‑90 (MQ=255) ttACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt > 2:408602/1‑90 (MQ=255) gATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAg > 2:199087/1‑90 (MQ=255) gATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAg > 2:376228/1‑90 (MQ=255) | AACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAG > NZ_CP009273/4368535‑4368700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |