Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,646,989	(A)5→4	intergenic (+23/‑332)	BW25113_RS18165 → / → slp	hypothetical protein/outer membrane lipoprotein Slp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,646,985	0	A	.	100.0%	100.7 / NA	24	intergenic (+19/‑336)	BW25113_RS18165/slp	hypothetical protein/outer membrane lipoprotein Slp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (6/18);  total (6/18)

AATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATAC  >  NZ_CP009273/3646898‑3647068                                                                                        |                                                                                    aaTATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCGCTGCCTAATGaaa                                                                                   <  1:28912/90‑1 (MQ=255) aaTATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGaaa                                                                                   <  2:72220/90‑1 (MQ=255) aaTATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGaaa                                                                                   <  1:310137/90‑1 (MQ=255) aaTATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGaaa                                                                                   >  2:252254/1‑90 (MQ=255)      cAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGaaaagata                                                                              <  1:396282/90‑5 (MQ=255)                   ttCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGt                                                                <  2:228179/90‑1 (MQ=255)                   ttCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGt                                                                <  1:3994/90‑1 (MQ=255)                       aTACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGgaa                                                            <  1:77245/90‑1 (MQ=255)                       aTACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGgaa                                                            <  1:31775/90‑1 (MQ=255)                       aTACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGgaa                                                            <  1:375679/90‑1 (MQ=255)                         aCGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGgaaat                                                          >  1:188072/1‑90 (MQ=255)                            agaCGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGgaaataga                                                       >  2:22237/1‑90 (MQ=255)                                gCTTAACCCAAAAAAAATGTCATACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGgaaatagaaata                                                   <  1:335581/90‑1 (MQ=255)                                     aCCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATc                                              <  2:195715/90‑1 (MQ=255)                                                             cTTATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCaaa                      <  1:123545/90‑1 (MQ=255)                                                               tATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGc                              <  1:55413/80‑1 (MQ=255)                                                               tATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGc                              >  2:55413/1‑80 (MQ=255)                                                               tATTTAAAGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATc                    >  1:31620/1‑90 (MQ=255)                                                                     aaGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTAcc              <  2:78312/90‑1 (MQ=255)                                                                     aaGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTAcc              <  2:117891/90‑1 (MQ=255)                                                                     aaGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTAcc              <  1:358024/90‑1 (MQ=255)                                                                     aaGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTAcc              <  1:22237/90‑1 (MQ=255)                                                                     aaGAGGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTAcc              <  1:150763/90‑1 (MQ=255)                                                                       gagGCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAg            <  2:31620/90‑1 (MQ=255)                                                                         ggCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGaa          >  1:223898/1‑90 (MQ=255)                                                                         ggCCACTGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGaa          >  2:56150/1‑90 (MQ=255)                                                                               tGCCTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATataat    <  2:165903/90‑1 (MQ=255)                                                                               tGCCTAATG‑AAAAGATATAAATTACGGGATTGAAATAGAAATACCAACAGGTAAAAAAATTCACTTACCAAATCATTACCAGAATataat    <  2:220522/90‑1 (MQ=255)                                                                                 ccTAATG‑AAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATAc  <  2:188072/90‑1 (MQ=255)                                                                                        |                                                                                    AATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATAC  >  NZ_CP009273/3646898‑3647068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑