Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	510,152	Δ1 bp	coding (164/459 nt)	ybbJ ←	NfeD family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	510,151	0	C	.	100.0%	19.9 / NA	6	coding (165/459 nt)	ybbJ	NfeD family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (3/3);  total (3/3)

CCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGC  >  NZ_CP009273/510103‑510212                                                 |                                                              ccacAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCACTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTa                     <  2:302723/90‑1 (MQ=255)       ccAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCAcc               <  2:226711/90‑1 (MQ=255)         aGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGc             >  2:179309/1‑90 (MQ=255)                gAGCAGCGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcg      >  1:404289/1‑90 (MQ=255)                  gcagcGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcgcc    <  1:193442/90‑1 (MQ=255)                    agcGTCAGGATGGCAAACATTACCCCTTG‑CACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcgccgc  >  1:111201/1‑90 (MQ=255)                                                 |                                                              CCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGC  >  NZ_CP009273/510103‑510212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑