Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,122,270	(A)6→5	coding (9/924 nt)	yceM →	Gfo/Idh/MocA family oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,122,265	0	A	.	83.3%	12.0 / ‑2.8	6	coding (4/924 nt)	yceM	Gfo/Idh/MocA family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (2/3);  minor base T (0/1);  total (2/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGAC  >  NZ_CP009273/1122197‑1122345                                                                     |                                                                                 ggAAATCGGAGTGGTAGAACTGAAACAGAGTCTTGATACGTGGCTTGCCCATCTGGGAGATTAAAGT‑TAAAAATTCCGTATCGGCGTAGt                                                            <  2:361838/90‑1 (MQ=255)                            gCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGc                                 >  2:338469/1‑90 (MQ=255)                              gTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGt                               >  2:274503/1‑90 (MQ=255)                                     ttCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTAcc                        <  1:215341/90‑1 (MQ=255)                                     ttCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTAcc                        <  1:274503/90‑1 (MQ=255)                                                           gaTTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGAc  <  2:247399/90‑1 (MQ=255)                                                                     |                                                                                 GGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGAC  >  NZ_CP009273/1122197‑1122345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑