Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,300,524	Δ1 bp	coding (504/1005 nt)	oppF →	murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,300,522	0	G	.	100.0%	33.1 / NA	9	coding (502/1005 nt)	oppF	murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (4/5);  total (4/5)

GAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGA  >  NZ_CP009273/1300444‑1300607                                                                               |                                                                                      gAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGc                                                                           <  1:51872/90‑1 (MQ=255)     gcgTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTAt                                                                       >  2:202609/1‑90 (MQ=255)            ggCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGAtt                                                                <  2:402323/90‑1 (MQ=255)                  gatgCTGAAAGTCGGGTTATTGCCTAGCCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                          <  2:81935/90‑1 (MQ=255)                                           aaCCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGa                                 >  1:197116/1‑90 (MQ=255)                                            aCCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGAt                                <  2:366809/90‑1 (MQ=255)                                                                cATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGgtgt            >  2:34651/1‑90 (MQ=255)                                                                       tctcTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCt     >  1:342367/1‑90 (MQ=255)                                                                          ctGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGa  <  2:35424/90‑1 (MQ=255)                                                                               |                                                                                      GAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGA  >  NZ_CP009273/1300444‑1300607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑