Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,753	(A)6→5	coding (377/435 nt)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,748	0	A	.	100.0%	41.5 / NA	11	coding (372/435 nt)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/6);  total (5/6)

CACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGA  >  NZ_CP009273/1613679‑1613831                                                                      |                                                                                    caccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGaa                                                                <  1:131522/90‑1 (MQ=255) caccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGaa                                                                <  2:104874/90‑1 (MQ=255)         gcgcggcAATATGTGAACAATGCCATCAATTAGTTGGCGAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAac                                                        <  2:315161/90‑1 (MQ=255)                    tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt                                             <  1:273152/90‑1 (MQ=255)                           aaTGCCATCAATTAGTTGGCCAGGGCCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa                                      >  2:24648/1‑90 (MQ=255)                            aTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAg                                     >  2:176519/1‑90 (MQ=255)                                 aTCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGt                                >  1:337591/1‑90 (MQ=255)                                 aTCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGt                                >  2:254591/1‑90 (MQ=255)                                                            aaGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt                                             <  1:224940/50‑1 (MQ=255)                                                            aaGAATTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt                                             >  2:224940/1‑50 (MQ=255)                                                               aaTTAAC‑AAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGa  <  2:54674/90‑1 (MQ=255)                                                                      |                                                                                    CACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGA  >  NZ_CP009273/1613679‑1613831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑