Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,417,788	(C)6→7	coding (69/120 nt)	BW25113_RS26130 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,417,782	1	.	C	100.0%	16.3 / NA	7	G25G (GGA→GGG)	BW25113_RS26130	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/7);  total (0/7)

CACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCC  >  NZ_CP009273/1417716‑1417854                                                                    |                                                                         cacaTCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGc                                                    <  2:125779/90‑1 (MQ=255)      cAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCCCCCCCGCCTCCGGCGTGTATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAAc                                               <  1:376617/90‑1 (MQ=11)      cAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAAc                                               <  2:125745/90‑1 (MQ=255)           cAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGat                                          <  2:129408/90‑1 (MQ=255)                                             cTCCGGCGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGc        <  2:227895/90‑1 (MQ=255)                                                   cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc  <  1:394622/90‑1 (MQ=255)                                                   cGTGGATAATGCAGCCTCCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGccc  <  2:253838/90‑1 (MQ=255)                                                                    |                                                                         CACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCT‑CCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCCGGGAACGACTGGTAATGATGCAGGCCC  >  NZ_CP009273/1417716‑1417854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑