| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 518,782 | 0 | T | G | 58.8% | 8.7 / 17.9 | 17 | G22G (GGT→GGG) | rhsD | rhs element protein RhsD |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (10/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGC > NZ_CP009273/518696‑518870 | aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGGCCCCTTTTCCCGGGGTCg > 2:110197/1‑90 (MQ=25)aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGGCCCCATGTCCAGGGtttg > 2:139248/1‑88 (MQ=25) aCTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCa < 1:71182/90‑1 (MQ=255) aaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCAGGGGTCGGGAGgggt > 2:111104/1‑90 (MQ=38) aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTa < 2:377210/90‑1 (MQ=255) atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGTGTAAGAATTGgcgcg > 1:114268/1‑90 (MQ=255) atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGACAGGGGTAAGAATTGgcgcg > 1:59923/1‑90 (MQ=25) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTTTCCCGGGGTCGGGAGGGGGAAGAAATTGGGCGcccc > 2:292925/1‑89 (MQ=18) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCCGGGGTCGGGCGGGGGAAAAAATGGCGCGcccc > 2:145746/1‑89 (MQ=18) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGt < 2:308789/62‑1 (MQ=255) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGt > 1:308789/1‑62 (MQ=255) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAAAAATGGCGGGcccc > 1:332684/1‑89 (MQ=21) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGGAAGAATTGGCGCGCCCa > 1:129946/1‑90 (MQ=25) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCACTGTCCAGGGGTCGGCAGGGGGAAGAATTTGGGGGcccc > 2:15836/1‑89 (MQ=21) cGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCAc < 1:292925/90‑1 (MQ=255) cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGgtg < 2:358728/90‑1 (MQ=255) aCTCAGTATGGCGGTCCCCTTGGCCAGGGTTCCCGAGGGGGAAAAAATGGCGGGCCCCCCCCCGGGGCGGGCGCGGGTCGTCcgggcggg > 2:112833/1‑90 (MQ=255) cAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAAAATTGGGGCGCCCCCCCGCGGGGGGGGGTCGGGGTGGCCGGGGGGGGTg > 2:114183/1‑90 (MQ=255) cATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAAccc < 1:295584/90‑1 (MQ=255) gTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgc < 2:380476/90‑1 (MQ=255) | ATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGC > NZ_CP009273/518696‑518870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |