| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 754,034 | 0 | G | T | 52.9% | 9.7 / 25.0 | 17 | intergenic (+173/‑128) | sdhB/sucA | succinate dehydrogenase iron‑sulfur subunit SdhB/2‑oxoglutarate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base G (7/1); new base T (0/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTATCCAC > NZ_CP009273/753948‑754123 | ttACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTGCCCCTGACACTAAGACAGttt > 1:54862/1‑90 (MQ=255) gTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTGCCCCTGACACTAAGACAGTTTTTAAAg > 1:291917/1‑90 (MQ=255) cgcggcccaTCGGGCAAAAAAAAAAAAGGCA‑GAAATTTTTAAAAACCCCCCCCTGACACAAAGACATTTTTTAAAGGTTCCTTCGCGAGc < 2:54862/90‑1 (MQ=255) gcagcgcaTCGGGCGAAAAAAAAAAATGCAGAAAATTTTAAAAAA‑CTCCCCCTGACACTAAGACATTTTTTAAAGGTTCCTTCGCGAGcc < 1:320530/90‑1 (MQ=255) agcgcaTCGGGGGAAAAAAAAAAATGCAGAAAATTTTAAAAAA‑CTCCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCAc < 1:306636/90‑1 (MQ=255) ggCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGAc > 1:13142/1‑90 (MQ=255) aaaaaaaaaATGCAGAAAATTTTAAAAAC‑CTCCCCCTGACACTAAGACATTTTTTAAAGGTTCCTCCGCGAGCCACTACGTAGACAagag < 1:44834/87‑1 (MQ=255) aTGCAGGAAATCTTTAAAAA‑CTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGt > 2:188500/1‑90 (MQ=255) gCAGGAAATTTTAAAAAC‑CTCCCCCTGACACTAAGACATTTTTAAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGa < 1:317297/90‑1 (MQ=255) aaaTCTTTAAAAA‑CTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAAcccc > 2:139675/1‑90 (MQ=255) ttCTTAAAAAC‑CCCCCCCTGACAAAAAGACATTTTTAAAGGGTCCCTTCGGGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCgg < 2:369493/89‑1 (MQ=255) cTTTAAAAA‑CTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGgca > 2:4300/1‑90 (MQ=255) tttAAAAACCTCCCCCTGACACAAAGACATTTTTAAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGgca < 1:202408/90‑1 (MQ=255) aaac‑CTCCCCCTGACACAAAGACATTTTTAAAAGGTTCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGgcacgcac < 1:202376/90‑1 (MQ=255) cTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCa > 2:204991/1‑90 (MQ=255) ccccTGACACAAAGACTTTTTTAAAAGTTTCCTTCGGGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACtg < 2:291917/90‑1 (MQ=255) gACACTAAGAATTTTTTTAAAGCTCCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGt < 2:53382/90‑1 (MQ=255) gTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTATCCAc > 2:197701/1‑90 (MQ=255) | TTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTAGTATCCAC > NZ_CP009273/753948‑754123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |