| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,241 | 0 | C | A | 54.5% | 2.1 / 11.7 | 11 | N29K (AAC→AAA) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base A (6/0); total (8/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCT‑GCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCTGTACCGCCATCATGATGTTCC > NZ_CP009273/2141155‑2141324 | gtgtTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGAc < 1:164476/90‑1 (MQ=255) gTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCAAGCGTGGCGGGAAAACGCATTTGCTAAAAAAGGAAAAAGACAACAcgcc‑gc > 2:133158/1‑90 (MQ=255) cAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAAACGCATTTGCTAAAAAAGGAAAACGACAGCACGCT‑GCTGCCTTCAATGCttt > 1:87278/1‑90 (MQ=255) cAGCAAACTGTTCAGTGGCGGGGATGCGTGAGGGGAAAACGCCTTTGGTAAAAAAAGAAAAAGAAAAAACGCC‑GCTTCCTTCAAATCttt > 2:327428/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGGGACGGGAAAACGCATTTGCTAAAAAAAGAAAAAGACAGCACGCT‑GCCGCCCTCAATGCTTTTCGCGcc > 2:72344/1‑90 (MQ=255) cTGTTCAGTGGCGGGCATTGGGGGCCGGAAAACGCATTTTCTTAAAAAAGAAAAAAACCCCACGCTGGCGGCCTT‑AATGCTTTTCGCGcc > 2:377535/1‑90 (MQ=255) gTTCAGTGGCGGTCATGCGTGACGGGAAACCGCATTTGCTAAAAAAGGAAAAAGAAAGCACGCT‑GCTGCCTTCAATGCTTTTCGCGccca > 2:204037/1‑88 (MQ=255) gTGGCGGTCATGCGGGACGGTAAACCGCATTTTCTAAAAAAGGAAAAAGACAGCACCCT‑GCTGCCTTCAATGCTTTTCGCGCCCACGCGt > 1:92413/1‑90 (MQ=255) cGCATTTGCTAAAAATGGAAAACGACAGCACGCT‑GCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCTGTAcc < 2:92413/90‑1 (MQ=255) cATTTGCTAAAAATGGAAAACGACAGCACGCT‑GCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCTGTACCGc > 2:244236/1‑90 (MQ=255) ggAAAACGACAGCACGCT‑GCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCTGTACCGCCATCATGATGTTcc < 1:352106/90‑1 (MQ=255) | GTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCT‑GCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGCTGTACCGCCATCATGATGTTCC > NZ_CP009273/2141155‑2141324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |