| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,463 | 0 | T | G | 53.8% | 2.7 / 10.4 | 13 | M671R (ATG→AGG) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (0/7); total (3/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAA > NZ_CP009273/2852377‑2852546 | acaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTAtggt > 2:54535/1‑90 (MQ=255) tCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGa > 2:163788/1‑90 (MQ=255) atcgCATCTTTCCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAATCCGCCAATAttt < 2:72977/90‑1 (MQ=255) tccccGCGCGGGGGCGGCAGAGGCCCTGCCGGCGGGGCCCTAAACTTTTTTGGTGGAGTTTACTAAAACCGCCAATATTTTACATAACGc < 2:187487/88‑1 (MQ=255) tACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGc > 1:204679/1‑90 (MQ=255) cgGCAGATGCCCTGGCGTCCGGGCGCTCAACTTTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTc < 1:335058/90‑1 (MQ=255) cgGCAGATGCCCGGGCGTCGGGGCGCTCACCTTTTAGGGTGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTc < 2:6748/90‑1 (MQ=255) gccAGATGACCGGGCGCCCGGCCCCTCACCTTTTAGGGGGGAGATGACGAAACCCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 2:281333/88‑1 (MQ=255) aCCGGGGGTCGGGGCGCTCAACTTTTAGGGGGGAGATGTCAGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTaa < 2:204679/90‑1 (MQ=255) cgggCGCCCGGGCCCCCAACCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATg < 1:396464/88‑1 (MQ=255) ccGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATgaga > 1:376815/1‑90 (MQ=255) gggCGCCAACCCTTTAGGGGGGAGATGAGGAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 2:357412/90‑1 (MQ=255) gccgcTCAACCTTTAGGGTGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCg < 2:376815/88‑1 (MQ=255) cttttAGGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGaa < 2:25926/88‑1 (MQ=255) | ACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAA > NZ_CP009273/2852377‑2852546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |