| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,837,579 | 0 | T | G | 55.0% | 9.9 / 16.5 | 20 | F86V (TTT→GTT) | adeD | adenine deaminase |
| Reads supporting (aligned to +/- strand): ref base T (4/5); new base G (11/0); total (15/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCC > NZ_CP009273/3837499‑3837657 | ttGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTTATg > 1:223508/1‑90 (MQ=255) gacaggTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGc < 1:307001/86‑1 (MQ=255) gCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCt > 1:337322/1‑90 (MQ=255) cGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGGGCCAGGGGTTTTTGATGCTcc > 2:326718/1‑89 (MQ=255) aGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCCCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGa > 1:12113/1‑90 (MQ=255) cacTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCATATTGAATCCAg > 1:161525/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCAc > 1:249271/1‑68 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCAc < 2:249271/68‑1 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCa < 2:274371/73‑1 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCatga > 1:114995/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTTTTGGTGCTCACCTGCATATTTAATCCAGCatga > 2:345383/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCCTATTGAATCCAGCatga > 2:376776/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCATATTGAATCCAGCatga > 2:146086/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCATATTGAATCCAGCatga > 1:345971/1‑90 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGAGCCAGGGTTTTTTTATGCTCACCTGCa > 1:274371/1‑73 (MQ=255) aTGCTCCGGCTTTGCAGCGGATTGATGCTCGAGGCGCAACGGCGGTGCCAGGGGTTTTTTATGCTCCCCCGCATATTGAATCCAGCatgg > 2:357409/1‑89 (MQ=255) ccGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACg > 2:286730/1‑90 (MQ=255) ttGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGGTTTTTGATGCTCACCCGCCTATTGAATCCAGCATGATGACGCCGGTc > 2:339268/1‑90 (MQ=255) cggcggTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCcgcgcg < 2:375374/90‑1 (MQ=255) cggTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCggac < 2:223508/90‑3 (MQ=255) | TTGCCGGTGTTGGCGCAGAATACACTGATGCTCCGGCTTTGCAGCGGATTGATGCTCGCGGCGCAACGGCGGTGCCAGGGTTTATTGATGCTCACCTGCATATTGAATCCAGCATGATGACGCCGGTCACTTTTGAAACCGCTACCCTGCCGCGCGGCC > NZ_CP009273/3837499‑3837657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |