| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,326,728 | 0 | T | C | 55.6% | 4.7 / 13.8 | 18 | I41V (ATT→GTT) | adiC | arginine/agmatine antiporter |
| Reads supporting (aligned to +/- strand): ref base T (5/3); new base C (0/10); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATT > NZ_CP009273/4326645‑4326813 | tGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGcc > 1:239633/1‑90 (MQ=255) aacaacATTTGGGCGTACCCCACCGAGCCCCCCAGTCCCCCGATAATCGCCCCCACCCACCAAAAAATGGCAACCCCGCCAGTAGAGGcc < 2:407517/90‑1 (MQ=255) aaCGACTTTTTGGCGTATCCCACCGAGACCCCCAGTCCCCCGATAACCGCCACCACCCATCCAAAAATGGAAATCCCGCCAGTAGAGGcc < 2:26137/90‑1 (MQ=255) taccccAGCGAGCCCCCCAGTCCCCCGATAACGCCCCCCAACCACCAAAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAAc < 2:311705/86‑1 (MQ=255) aTCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGaaaa > 1:382756/1‑90 (MQ=255) acccccAGGGCACCAATAACCGCCCCCACCCACCAAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCt < 2:382756/88‑1 (MQ=255) gCCCCAGTGCCCCAATAATCGCCCCCAACCACCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg < 1:140597/90‑1 (MQ=255) cccccAGTCCACGAAAAACCGCCCCCACCCACCCAAAAAGGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg < 2:113991/89‑1 (MQ=255) cccccAGGGCAACGATAACCGCCCCCACCCACCAAAAAATGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg < 2:205320/89‑1 (MQ=255) ccccAGTCCACCGATAACCGCCCCCAACCACCAAAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGa < 2:41985/90‑1 (MQ=255) cccAGTGCCCCGAAAATCGCCCCCAACCCTCCAAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAc < 1:201497/90‑1 (MQ=255) cccAGTGCACCGAAAATCGTCACCAACCATCAAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAc < 2:46234/90‑1 (MQ=255) gcccccGATAACCGCAACCACCCACCCAAAAACGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCat < 1:304775/86‑1 (MQ=255) gccACCAACCATCAAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGac < 1:357761/88‑1 (MQ=255) acACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGaca > 2:368497/2‑90 (MQ=255) aTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTa < 2:101605/37‑1 (MQ=255) aTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTa > 1:101605/1‑37 (MQ=255) gCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAtt > 1:168398/1‑90 (MQ=255) | TGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATT > NZ_CP009273/4326645‑4326813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |