Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,741,778	A→T	Y153F (TAC→TTC)	ypjD →	inner membrane protein YpjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,741,778	0	A	T	100.0%	32.2 / NA	10	Y153F (TAC→TTC)	ypjD	inner membrane protein YpjD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/3);  total (7/3)

TATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATG  >  NZ_CP009273/2741709‑2741848                                                                      |                                                                       tATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGc                                                    >  1:80953/1‑90 (MQ=255)                     aCGCTAATCATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATg                                >  1:186514/1‑90 (MQ=255)                                   cgccCTGTACGCGCTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAg                  <  2:213910/90‑1 (MQ=255)                                    gccCTGTACGCGCTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGt                 >  1:210419/1‑90 (MQ=255)                                    gccCTGTACGCGCTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGt                 >  1:285912/1‑90 (MQ=255)                                    gccCTGTACGCGCTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGt                 >  2:229880/1‑90 (MQ=255)                                             gcgcTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGt        <  1:229880/90‑1 (MQ=255)                                              cgcTGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTa       >  2:15638/1‑90 (MQ=255)                                                 tGCAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTaaaa    <  2:403703/90‑1 (MQ=255)                                                   cAACTGGCGTGGATTGATTTCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATg  >  1:47829/1‑90 (MQ=255)                                                                      |                                                                       TATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATG  >  NZ_CP009273/2741709‑2741848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑