Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,987,546	T→C	I83I (ATT→ATC)	BW25113_RS14845 →	lytic transglycosylase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,987,546	0	T	C	100.0%	49.5 / NA	16	I83I (ATT→ATC)	BW25113_RS14845	lytic transglycosylase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/3);  total (13/3)

GAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGA  >  NZ_CP009273/2987477‑2987631                                                                      |                                                                                      gAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTgg                                                                   >  1:282630/1‑90 (MQ=255)                  aCAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAAc                                                  >  1:280942/1‑90 (MQ=255)                  aCAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAAc                                                  >  1:367472/1‑90 (MQ=255)                     atatAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACgag                                               >  1:282428/1‑90 (MQ=255)                       ataAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGAt                                             >  2:202988/1‑90 (MQ=255)                          aaGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTg                                          <  2:359661/90‑1 (MQ=255)                             agTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTa                                       >  2:398058/1‑90 (MQ=255)                              gTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAg                                      >  2:259162/1‑90 (MQ=255)                                ttAGATTATTGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGAt                                    <  1:242603/90‑1 (MQ=255)                                        ttGGTTAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTg                            >  1:151506/1‑90 (MQ=255)                                            ttAATGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTg                        <  2:239610/90‑1 (MQ=255)                                               aTGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAAc                     >  1:129253/1‑90 (MQ=255)                                               aTGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAAc                     >  2:262486/1‑90 (MQ=255)                                               aTGCAGATAAACCAGATGCATATCCCCCTGTTAAAAAAACGAGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTAGTCTGAAc                     >  2:72030/1‑90 (MQ=255)                                                    gATAAACCAGATGCATATCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTaa                >  1:287727/1‑90 (MQ=255)                                                                  atatCCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCgaga  >  2:334918/1‑90 (MQ=255)                                                                      |                                                                                      GAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGA  >  NZ_CP009273/2987477‑2987631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑