Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,291,696	T→G	L170F (TTA→TTC)	yraR ←	NAD(P)H‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,291,696	0	T	G	100.0%	40.8 / NA	13	L170F (TTA→TTC)	yraR	NAD(P)H‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/5);  total (8/5)

GTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGG  >  NZ_CP009273/3291610‑3291771                                                                                       |                                                                            gTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAAc                                                                          >  2:150791/1‑90 (MQ=255)                 cAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAgcggcg                                                          >  2:209374/1‑90 (MQ=255)                       gCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCaaaaa                                                    >  1:296258/1‑90 (MQ=255)                        cATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAg                                                   >  1:160877/1‑90 (MQ=255)                                        aTGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGc                                   >  1:152637/1‑90 (MQ=255)                                          gACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTg                                 <  1:5087/90‑1 (MQ=255)                                            cccGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGtt                               >  1:79918/1‑90 (MQ=255)                                               gcgcCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGc                            >  2:403943/1‑90 (MQ=255)                                                      gTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATc                     <  2:121517/90‑1 (MQ=255)                                                      gTCTCTCGCATCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATc                     <  2:186916/90‑1 (MQ=255)                                                               aTCAATGGATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTaa            >  1:92851/1‑90 (MQ=255)                                                                      gATTTCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGAc     <  1:269605/90‑1 (MQ=255)                                                                         ttCCAGTTACCTGGGAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACggg  <  2:317545/90‑1 (MQ=255)                                                                                       |                                                                            GTCACGCCTTCATGCTCAGGCCGCATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGG  >  NZ_CP009273/3291610‑3291771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑