Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,642,054	A→G	E56G (GAA→GGA)	arsR →	As(III)‑sensing metalloregulatory transcriptional repressor ArsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,642,054	0	A	G	100.0%	124.3 / NA	36	E56G (GAA→GGA)	arsR	As(III)‑sensing metalloregulatory transcriptional repressor ArsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

CTGGGAGAGTTATGCGTCTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGAAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCATATTCCAGCATGGGCGGCGAAA  >  NZ_CP009273/3641969‑3642136                                                                                      |                                                                                   cTGGGAGAGTTATGCGTCTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGc                                                                                <  1:217814/90‑1 (MQ=255)      agagTTATGCGTCTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCt                                                                           <  1:120044/90‑1 (MQ=255)      agagTTATGCGTCTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCt                                                                           <  1:317694/90‑1 (MQ=255)         gTTATGCGTCTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTAtt                                                                        >  2:8973/1‑90 (MQ=255)                 tCTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGAcc                                                                <  1:71572/90‑1 (MQ=255)                  cTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCg                                                               >  1:36467/1‑90 (MQ=255)                       aTCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCgcaagc                                                          <  1:130475/90‑1 (MQ=255)                          tctGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCgcaagc                                                          >  2:384720/1‑87 (MQ=255)                          tctGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCgcaagc                                                          <  1:384720/87‑1 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGc                                                                                <  2:351362/57‑1 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGc                                                                                >  1:351362/1‑57 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTgg                                               >  1:209809/1‑90 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTgg                                               >  1:132214/1‑90 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTgg                                               >  1:178357/1‑90 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTgg                                               >  1:243778/1‑90 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTg                                                >  1:268034/1‑89 (MQ=255)                                  gCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTg                                                <  2:268034/89‑1 (MQ=255)                                    tctcGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGt                                             <  2:401274/90‑1 (MQ=255)                                       cGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCa                                          >  2:109541/1‑90 (MQ=255)                                            aGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCgcaagca                                                         >  1:188693/1‑70 (MQ=255)                                            aGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCgcaagca                                                         <  2:188693/70‑1 (MQ=255)                                            aGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTAc                                      <  2:1577/89‑1 (MQ=255)                                            aGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTAc                                      >  1:1577/1‑89 (MQ=255)                                                   gCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATc                              <  1:16010/90‑1 (MQ=255)                                                         gATCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCa                        <  1:94145/90‑1 (MQ=255)                                                          aTCTCCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCat                       <  2:132214/90‑1 (MQ=255)                                                             tcCCGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCATAtt                    >  1:244736/1‑90 (MQ=255)                                                              cccGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCtt                                 >  2:26798/1‑76 (MQ=255)                                                              cccGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCtt                                 <  1:26798/76‑1 (MQ=255)                                                              cccGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCAc                            >  1:391018/1‑81 (MQ=255)                                                              cccGCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCAc                            <  2:391018/81‑1 (MQ=255)                                                                 gCCACCTGGCATTGCTGCGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCATATTCCAg                >  1:310337/1‑90 (MQ=255)                                                                              gctgcGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCATATTCCAGCATGGGCGGCGaa   >  1:131447/1‑90 (MQ=255)                                                                               ctgcGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCATATTCCAGCATGGGCGGCGaaa  <  2:392742/90‑1 (MQ=255)                                                                                  cGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTAc                                      <  2:373970/51‑1 (MQ=255)                                                                                  cGTGGAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTAc                                      >  1:373970/1‑51 (MQ=255)                                                                                      |                                                                                   CTGGGAGAGTTATGCGTCTGCGATCTCTGCACTGCTCTCGACCAGTCGCAGCCCAAGATCTCCCGCCACCTGGCATTGCTGCGTGAAAGCGGGCTATTGCTGGACCGCAAGCAAGGTAAGTGGGTTCATTACCGCTTATCACCGCATATTCCAGCATGGGCGGCGAAA  >  NZ_CP009273/3641969‑3642136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑